Click image to see more details
Product Info Summary
| SKU: | A00866-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ADA Antibody Picoband®
View all Adenosine Deaminase/ADA Antibodies
SKU/Catalog Number
A00866-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADA Antibody Picoband® catalog # A00866-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00866-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat ADA recombinant protein (Position: A2-H241).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00866-2 is reactive to Ada in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.764kDa
Background of Adenosine Deaminase/ADA
Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00866-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat brain tissue, rat liver tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue, mouse NIH/3T3 whole cell
IHC: rat spleen tissue, mouse intestine tissue, rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADA using anti-ADA antibody (A00866-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody (Catalog # A00866-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.
Click image to see more details
Figure 2. IHC analysis of ADA using anti-ADA antibody (A00866-2). ADA was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADA Antibody (A00866-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ADA using anti-ADA antibody (A00866-2). ADA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADA Antibody (A00866-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of ADA using anti-ADA antibody (A00866-2).
ADA was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADA Antibody (A00866-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ada (Source: Uniprot.org, NCBI)
Gene Name
Ada
Full Name
Adenosine deaminase
Weight
40.764kDa
Superfamily
metallo-dependent hydrolases superfamily
Alternative Names
Adenosine deaminase ADA ADA1 adenosine deaminase adenosine deaminase|adenosine aminohydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ada, check out the Ada Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ada: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADA Antibody Picoband® (A00866-2)
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Customer Q&As
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16 Customer Q&As for Anti-ADA Antibody Picoband®
Question
I see that the anti-ADA antibody A00866-2 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-03
Question
Does anti-ADA antibody A00866-2 work for ELISA with b-cell lymphoma?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
According to the expression profile of b-cell lymphoma, ADA is highly expressed in b-cell lymphoma. So, it is likely that anti-ADA antibody A00866-2 will work for ELISA with b-cell lymphoma.
Boster Scientific Support
Answered: 2020-01-10
Question
We were well pleased with the WB result of your anti-ADA antibody. However we have observed positive staining in duodenum cell membrane using this antibody. Is that expected? Could you tell me where is ADA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
From what I have seen in literature, duodenum does express ADA. Generally ADA expresses in cell membrane. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2019-11-19
Question
My question regarding product A00866-2, anti-ADA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00866-2 anti-ADA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-13
Question
See below the WB image, lot number and protocol we used for b-cell lymphoma using anti-ADA antibody A00866-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
We have seen staining in rat thymus. Are there any suggestions? Is anti-ADA antibody supposed to stain thymus positively?
A. Yang
Verified customer
Asked: 2019-08-27
Answer
From literature thymus does express ADA. From Uniprot.org, ADA is expressed in duodenum, thymus, brain placenta, b-cell lymphoma, leukemic t-cell, among other tissues. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2019-08-27
Question
I was wanting to use your anti-ADA antibody for ELISA for rat b-cell lymphoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat b-cell lymphoma identification?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, A00866-2 anti-ADA antibody has been validated for ELISA, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat b-cell lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Is this A00866-2 anti-ADA antibody reactive to the isotypes of ADA?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
The immunogen of A00866-2 anti-ADA antibody is E. coli-derived rat ADA recombinant protein (Position: A2-H241). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-01
Question
Is a blocking peptide available for product anti-ADA antibody (A00866-2)?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
We do provide the blocking peptide for product anti-ADA antibody (A00866-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-20
Question
We are currently using anti-ADA antibody A00866-2 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
The anti-ADA antibody (A00866-2) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-11
Question
Our lab want to know about to test anti-ADA antibody A00866-2 on rat b-cell lymphoma for research purposes, then I may be interested in using anti-ADA antibody A00866-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
The products we sell, including anti-ADA antibody A00866-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-03
Question
Would A00866-2 anti-ADA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Moore
Verified customer
Asked: 2018-05-25
Answer
As indicated on the product datasheet, A00866-2 anti-ADA antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell lymphoma using anti-ADA antibody A00866-2. Let me know if you need anything else.
C. Bhatt
Verified customer
Asked: 2017-11-29
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-29
Question
Do you have a BSA free version of anti-ADA antibody A00866-2 available?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADA antibody A00866-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-22
Question
We ordered your anti-ADA antibody for IHC on brain placenta in the past. I am using mouse, and I plan to use the antibody for WB next. I am interested in examining brain placenta as well as leukemic t-cell in our next experiment. Could give a recommendation on which antibody would work the best for WB?
A. Banerjee
Verified customer
Asked: 2015-04-28
Answer
I looked at the website and datasheets of our anti-ADA antibody and it seems that A00866-2 has been validated on mouse in both IHC and WB. Thus A00866-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-28
Question
We want using your anti-ADA antibody for purine nucleotide salvage studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
G. Carter
Verified customer
Asked: 2015-02-06
Answer
Thank you for your inquiry. This A00866-2 anti-ADA antibody is validated on mouse ovary tissue, testis tissue, rat stomach tissue, intestine tissue, brain tissue, spleen tissue, human placenta tissue. It is guaranteed to work for ELISA, IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-02-06
