Anti-ODF3 Antibody Picoband™

Product Name

Anti-ODF3 Antibody Picoband™

View all Odf3 Antibodies

SKU/Catalog Number

A14065-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-ODF3 Antibody Picoband™ catalog # A14065-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ODF3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14065-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ODF3 recombinant protein (Position: S25-E254).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14065-1 is reactive to Odf3 in Human, Mouse

Applications

A14065-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

64099 MW

Background of Odf3

ODF3 is a component of sperm flagella outer dense fibers, which add stiffness, elastic recoil, and protection against shearing forces during sperm movement.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ODF3 using anti-ODF3 antibody (A14065-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF3 antigen affinity purified polyclonal antibody (Catalog # A14065-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF3 at approximately 28 kDa. The expected band size for ODF3 is at 28,30 kDa.

Gene/Protein Information For Odf3 (Source: Uniprot.org, NCBI)

Alternative Names

CT135; hSHIPPO; outer dense fiber of sperm tails 3; outer dense fiber of sperm tails protein 3; outer dense fiber protein 3

For more info on Odf3, check out the Odf3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Odf3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Odf3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis