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Product Info Summary
| SKU: | A14065-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ODF3 Antibody Picoband®
SKU/Catalog Number
A14065-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ODF3 Antibody Picoband® catalog # A14065-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ODF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14065-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ODF3 recombinant protein (Position: S25-E254).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14065-1 is reactive to ODF3 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
64099 MW
Background of Odf3
ODF3 is a component of sperm flagella outer dense fibers, which add stiffness, elastic recoil, and protection against shearing forces during sperm movement.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14065-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse testis tissue
FCM: CACO-2 cell, U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ODF3 using anti-ODF3 antibody (A14065-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF3 antigen affinity purified polyclonal antibody (Catalog # A14065-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF3 at approximately 28 kDa. The expected band size for ODF3 is at 28,30 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ODF3 (Source: Uniprot.org, NCBI)
Gene Name
ODF3
Full Name
Outer dense fiber protein 3
Weight
64099 MW
Superfamily
ODF3 family
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ODF3, check out the ODF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ODF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ODF3 Antibody Picoband® (A14065-1)
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