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Product Info Summary
| SKU: | A00444 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RORC phospho S203 Antibody
SKU/Catalog Number
A00444
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-RORC phospho S203 Antibody (Catalog # A00444). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. This product is stable at 4°C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-RORC phospho S203 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00444)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Affinity purified Anti-receptor ROR gamma pS203 antibody was prepared from whole rabbit serum produced by repeated immunizations with an internal synthetic peptide phosphorylated at the serine 203 position of mouse receptor ROR gamma protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00444 is reactive to RORC in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
10085 MW
Background of RORC
Anti-Nuclear receptor ROR gamma pS203 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Nuclear receptor ROR gamma pS203 antibody detects mouse receptor ROR gamma phosphorylated at the serine 203 position. RAR-related orphan receptor gamma is a member of the nuclear receptor family of transcription factors. (ROR-gamma) is a key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism. ROR-gamma regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. It is also involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism and is a negative regulator of adipocyte differentiation. It controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. Isoform 2, ROR-gamma is essential for lymphoid organogenesis, in particular lymph nodes and Peyer's patches. ROR-gamma also plays an important regulatory role in thymopoiesis, and in inhibiting apoptosis of undifferentiated T cells. Anti-nuclear receptor ROR gamma pS203 is ideal for researcher's interested in autoimmune diseases such as psoriasis and rheumatoid arthritis, circadian rhythms, and immune system disorders.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00444 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 5 µg/ml
WB: 1 ug/mL
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rorc using anti-Rorc antibody (A00444).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rorc antigen affinity purified polyclonal antibody (Catalog # A00444) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For RORC (Source: Uniprot.org, NCBI)
Gene Name
RORC
Full Name
Nuclear receptor ROR-gamma
Weight
10085 MW
Superfamily
nuclear hormone receptor family
Alternative Names
receptor ROR gamma pS203, Nuclear receptor ROR-gamma, Nr1f3, RAR-related orphan receptor C, Nuclear receptor RZR-gamma, Thymus orphan receptor, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma, TOR, Thor, Rorg, Rorc RORC IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR RAR related orphan receptor C nuclear receptor ROR-gamma|RAR-related orphan nuclear receptor variant 2|nuclear receptor RZR-gamma|nuclear receptor subfamily 1 group F member 3|retinoic acid-binding receptor gamma|retinoid-related orphan receptor gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RORC, check out the RORC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RORC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RORC phospho S203 Antibody (A00444)
Hello CJ!
A00444 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation
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Customer Q&As
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5 Customer Q&As for Anti-RORC phospho S203 Antibody
Question
I see that the anti-Nuclear receptor ROR gamma pS203 antibody A00444 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-11
Question
I was wanting to use your anti-Nuclear receptor ROR gamma pS203 antibody for WB for mouse lymph node on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lymph node identification?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
You can see on the product datasheet, A00444 anti-Nuclear receptor ROR gamma pS203 antibody has been tested for ELISA, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lymph node in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
We are currently using anti-Nuclear receptor ROR gamma pS203 antibody A00444 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-29
Answer
The anti-Nuclear receptor ROR gamma pS203 antibody (A00444) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-29
Question
Please see the WB image, lot number and protocol we used for lymph node using anti-Nuclear receptor ROR gamma pS203 antibody A00444. Please let me know if you require anything else.
N. Taylor
Verified customer
Asked: 2017-12-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-25
Question
I have a question about product A00444, anti-Nuclear receptor ROR gamma pS203 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Patel
Verified customer
Asked: 2016-06-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00444 anti-Nuclear receptor ROR gamma pS203 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-06-30
