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Product Info Summary
| SKU: | M04626 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nuclear Matrix Protein p84 Monoclonal Antibody
View all hHpr1-p84-Thoc1 Antibodies
SKU/Catalog Number
M04626
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nuclear Matrix Protein p84 Monoclonal Antibody catalog # M04626. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nuclear Matrix Protein p84 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04626)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACFA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nuclear Matrix Protein p84 Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04626 is reactive to THOC1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated molecular weight
83166 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04626 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
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Western blot analysis of Nuclear Matrix Protein p84 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For THOC1 (Source: Uniprot.org, NCBI)
Gene Name
THOC1
Full Name
THO complex subunit 1
Weight
83166 MW
Alternative Names
Glycine--tRNA ligase;3.6.1.17 ;6.1.1.14 ;Diadenosine tetraphosphate synthetase;AP-4-A synthetase;Glycyl-tRNA synthetase;GlyRS;GARS; THOC1 HPR1, P84, P84N5 THO complex 1 THO complex subunit 1|hTREX84|nuclear matrix protein p84|tho1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THOC1, check out the THOC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THOC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nuclear Matrix Protein p84 Monoclonal Antibody (M04626)
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No publications found for M04626
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Customer Q&As
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3 Customer Q&As for Anti-Nuclear Matrix Protein p84 Monoclonal Antibody
Question
Would anti-Nuclear Matrix Protein p84 Monoclonal antibody M04626 work for WB with bone marrow?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
According to the expression profile of bone marrow, THOC1 is highly expressed in bone marrow. So, it is likely that anti-Nuclear Matrix Protein p84 Monoclonal antibody M04626 will work for WB with bone marrow.
Boster Scientific Support
Answered: 2020-02-26
Question
Would M04626 anti-Nuclear Matrix Protein p84 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can see on the product datasheet, M04626 anti-Nuclear Matrix Protein p84 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-09
Question
We are currently using anti-Nuclear Matrix Protein p84 Monoclonal antibody M04626 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
S. Parker
Verified customer
Asked: 2015-11-18
Answer
The anti-Nuclear Matrix Protein p84 Monoclonal antibody (M04626) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-18
