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Product Info Summary
| SKU: | A00078-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NRF2/NFE2L2 Antibody Picoband®
SKU/Catalog Number
A00078-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NRF2/NFE2L2 Antibody Picoband® catalog # A00078-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NRF2/NFE2L2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00078-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NRF2/NFE2L2 recombinant protein (Position: E67-D601).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00078-2 is reactive to NFE2L2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
100-110 kDa
Calculated molecular weight
68 kDa
Background of Nrf2
Nuclear factor erythroid 2-related factor 2 (NRF2), also known as nuclear factor erythroid-derived 2-like 2, is a transcription factor that in humans is encoded by the NFE2L2 gene. This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00078-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human U20S whole cell, human U20S(+MG132) whole cell
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NRF2/NFE2L2 using anti-NRF2/NFE2L2 antibody (A00078-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human U20S(+MG132) whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRF2/NFE2L2 antigen affinity purified polyclonal antibody (Catalog # A00078-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRF2/NFE2L2 at approximately 100-110 kDa. The expected band size for NRF2/NFE2L2 is at 68 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-NRF2/NFE2L2 antibody (A00078-2).
Overlay histogram showing RT4 cells stained with A00078-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRF2/NFE2L2 Antibody (A00078-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NFE2L2 (Source: Uniprot.org, NCBI)
Gene Name
NFE2L2
Full Name
Nuclear factor erythroid 2-related factor 2
Weight
68 kDa
Superfamily
bZIP family
Alternative Names
HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2 ihc; Nrf2 immunofluorescence; Nrf2 immunohistochemistry; Nrf2 polyclonal; Nrf2 rat; Nrf2 western blot; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2 NFE2L2 HEBP1, IMDDHH, NRF2, Nrf-2 nuclear factor, erythroid 2 like 2 nuclear factor erythroid 2-related factor 2|nuclear factor erythroid-derived 2-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFE2L2, check out the NFE2L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFE2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NRF2/NFE2L2 Antibody Picoband® (A00078-2)
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