Product Info Summary
SKU: | PB9298 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NR5A2/LRH1 Antibody Picoband™
View all LRH-1/NR5A2 Antibodies
SKU/Catalog Number
PB9298
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NR5A2/LRH1 Antibody Picoband™ catalog # PB9298. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR5A2/LRH1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9298)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NR5A2 recombinant protein (Position: K44-R237). Human NR5A2 shares 95% amino acid (aa) sequence identity with both mouse and rat NR5A2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9298 is reactive to NR5A2 in Human
Applications
PB9298 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
61 kDa
Calculated molecular weight
61331 MW
Background of LRH-1/NR5A2
NR5A2 (nuclear receptor subfamily 5, group A, member 2) also known as liver receptor homolog-1 (LRH-1) is a protein that in humans is encoded by the NR5A2 gene. LRH-1 is a member of the nuclear receptor family of intracellulartranscription factors. LRH-1 plays a critical role in the regulation of development,cholesterol transport, bile acid homeostasis andsteroidogenesis. LRH-1 is important for maintaining pluripotence of stem cells during embryonic development. Liver receptor homolog-1 has been shown to interact with the small heterodimer partner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
![pb9298 1 WB anti nr5a2 lrh1 picoband antibody pb9298 1 WB anti nr5a2 lrh1 picoband antibody](https://www.bosterbio.com/media/catalog/product/antibody/pb9298-1-WB-anti-nr5a2-lrh1-picoband-antibody.jpg)
Click image to see more details
Anti-NR5A2 Picoband antibody, PB9298, Western blotting
All lanes: Anti NR5A2 (PB9298) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
Protein Target Info & Infographic
Gene/Protein Information For NR5A2 (Source: Uniprot.org, NCBI)
Gene Name
NR5A2
Full Name
Nuclear receptor subfamily 5 group A member 2
Weight
61331 MW
Superfamily
nuclear hormone receptor family
Alternative Names
Alpha-1-fetoprotein transcription factor; B1-binding factor; b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus; B1F; B1F2; fetoprotein-alpha 1 (AFP) transcription factor; FTFLRH1; FTZ-F1 beta; FTZ-F1; FTZ-F1beta; HB1F; hB1F-2; hB1FCYP7A promoter-binding factor; Hepatocytic transcription factor; Liver receptor homolog 1; liver receptor homolog-1; LRH1; LRH-1; LRH-1CPF; NR5A2; nuclear receptor NR5A2; nuclear receptor subfamily 5 group A member 2; nuclear receptor subfamily 5, group A, member 2 NR5A2 B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2 nuclear receptor subfamily 5 group A member 2 nuclear receptor subfamily 5 group A member 2|CYP7A promoter-binding factor|b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus|fetoprotein-alpha 1 (AFP) transcription factor|hepatocytic transcription factor hB1F-3|liver nuclear receptor homolog-1 variant 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR5A2, check out the NR5A2 Infographic
![NR5A2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NR5A2/LRH1 Antibody Picoband™ (PB9298)
Hello CJ!
PB9298 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhao W, Wen Hx, Zheng Hl, Sun Sx, Sun Dj, Ni J. J Ethnopharmacol. 2011 Mar 8;134(1):122-9. Doi: 10.1016/J.Jep.2010.11.073. Epub 2010 Dec 4. Action Mechanism Of Zuo Gui Yin Decoction'S Promotion On Estradiol Production In Rats During The Peri-Menop...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NR5A2/LRH1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NR5A2/LRH1 Antibody Picoband™
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-NR5A2/LRH1 Antibody Picoband™
Question
We are currently using anti-NR5A2/LRH1 antibody PB9298 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
D. Li
Verified customer
Asked: 2013-10-30
Answer
The anti-NR5A2/LRH1 antibody (PB9298) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-30