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Product Info Summary
| SKU: | M00494 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody
SKU/Catalog Number
M00494
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody catalog # M00494. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00494)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAOB-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NQO1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00494 is reactive to NQO1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
27,31 kDa
Calculated molecular weight
30868 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00494 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC:1-50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
ICC/IF: MCF-7 cell
IHC: mouse stomach tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NQO1 using anti-NQO1 antibody (M00494).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified monoclonal antibody (Catalog # M00494) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 27,31 kDa. The expected band size for NQO1 is at 31 kDa.
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Figure 2. IHC analysis of NQO1 using anti-NQO1 antibody (M00494).
NQO1 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-NQO1 Antibody (M00494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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All lanes use the Antibody at 1:1w dilution for 1 hour at room temperature.
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Immunofluorescent analysis of MCF-7 cells, using NQO1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NQO1 (Source: Uniprot.org, NCBI)
Gene Name
NQO1
Full Name
NAD(P)H dehydrogenase [quinone] 1
Weight
30868 MW
Superfamily
NAD(P)H dehydrogenase (quinone) family
Alternative Names
NAD (P)H dehydrogenase [quinone] 1;1.6.5.2;Azoreductase;DT-diaphorase;DTD;Menadione reductase;NAD (P)H:quinone oxidoreductase 1;Phylloquinone reductase;Quinone reductase 1;QR1;NQO1;DIA4, NMOR1; NQO1 DHQU, DIA4, DTD, NMOR1, NMORI, QR1 NAD(P)H quinone dehydrogenase 1 NAD(P)H dehydrogenase [quinone] 1|DT-diaphorase|NAD(P)H dehydrogenase, quinone 1|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H:menadione oxidoreductase 1|NAD(P)H:quinone oxidoreductase 1|NAD(P)H:quinone oxireductase|azoreductase|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase-4|dioxin-inducible 1|menadione reductase|phylloquinone reductase|quinone reductase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NQO1, check out the NQO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NQO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody (M00494)
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