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Product Info Summary
| SKU: | M00033 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Notch1 Monoclonal Antibody
SKU/Catalog Number
M00033
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Notch1 Monoclonal Antibody catalog # M00033. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Notch1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00033)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BGA-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00033 is reactive to NOTCH1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
110 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00033 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500
Positive Control
WB: Human 293T whole cell, Human K562 whole cell, Human Jurkat whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: human liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NOTCH1 using anti-NOTCH1 antibody (M00024-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human 293T whole cell lysates,
Lane 2: Human K562 whole cell lysates,
Lane 3: Human Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH1 antigen affinity purified monoclonal antibody (Catalog # M00024-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOTCH1 at approximately 110 kDa. The expected band size for NOTCH1 is at 273 kDa.
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Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .
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Immunofluorescent analysis of HeLa cells, using Notch1 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NOTCH1 (Source: Uniprot.org, NCBI)
Gene Name
NOTCH1
Full Name
Neurogenic locus notch homolog protein 1
Weight
88068 MW
Superfamily
NOTCH family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; NOTCH1 AOS5, AOVD1, TAN1, hN1 notch receptor 1 neurogenic locus notch homolog protein 1|Notch homolog 1, translocation-associated|notch 1|translocation-associated notch protein TAN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOTCH1, check out the NOTCH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOTCH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Notch1 Monoclonal Antibody (M00033)
Hello CJ!
M00033 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Involvement of Notch2 in all%u2011trans retinoic acid%u2011induced inhibition of mouse embryonic palate mesenchymal cell proliferation
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Customer Q&As
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16 Customer Q&As for Anti-Notch1 Monoclonal Antibody
Question
We are interested in using your anti-Notch1 Monoclonal antibody for arterial endothelial cell differentiation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you for your inquiry. This M00033 anti-Notch1 Monoclonal antibody is tested on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-15
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Notch1 Monoclonal antibody M00033. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
My question regarding product M00033, anti-Notch1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00033 anti-Notch1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-11
Question
Would M00033 anti-Notch1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
As indicated on the product datasheet, M00033 anti-Notch1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-10
Question
Is this M00033 anti-Notch1 Monoclonal antibody reactive to the isotypes of NOTCH1?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
The immunogen of M00033 anti-Notch1 Monoclonal antibody is A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-07
Question
Does anti-Notch1 Monoclonal antibody M00033 work for IHC with brain?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
According to the expression profile of brain, NOTCH1 is highly expressed in brain. So, it is likely that anti-Notch1 Monoclonal antibody M00033 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-Notch1 Monoclonal antibody M00033 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The anti-Notch1 Monoclonal antibody (M00033) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-30
Question
Do you have a BSA free version of anti-Notch1 Monoclonal antibody M00033 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Notch1 Monoclonal antibody M00033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
I see that the anti-Notch1 Monoclonal antibody M00033 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-11
Question
I was wanting to use your anti-Notch1 Monoclonal antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
You can see on the product datasheet, M00033 anti-Notch1 Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Notch1 Monoclonal antibody M00033. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-31
Question
Is a blocking peptide available for product anti-Notch1 Monoclonal antibody (M00033)?
Verified Customer
Verified customer
Asked: 2018-05-16
Answer
We do provide the blocking peptide for product anti-Notch1 Monoclonal antibody (M00033). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-16
Question
We want to test anti-Notch1 Monoclonal antibody M00033 on human brain for research purposes, then I may be interested in using anti-Notch1 Monoclonal antibody M00033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Singh
Verified customer
Asked: 2016-11-25
Answer
The products we sell, including anti-Notch1 Monoclonal antibody M00033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-25
Question
We have been able to see staining in mouse aortic endothelium. What should we do? Is anti-Notch1 Monoclonal antibody supposed to stain aortic endothelium positively?
A. Zhao
Verified customer
Asked: 2015-10-16
Answer
From what I have seen in literature aortic endothelium does express NOTCH1. From what I have seen in Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2015-10-16
Question
We were content with the WB result of your anti-Notch1 Monoclonal antibody. However we have seen positive staining in brain notch 1 intracellular domain: nucleus using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?
J. Yang
Verified customer
Asked: 2014-07-24
Answer
From what I have seen in literature, brain does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648
Boster Scientific Support
Answered: 2014-07-24
Question
We ordered your anti-Notch1 Monoclonal antibody for WB on cervix carcinoma in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. I am interested in examining cervix carcinoma as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
C. Wu
Verified customer
Asked: 2013-04-08
Answer
I looked at the website and datasheets of our anti-Notch1 Monoclonal antibody and I see that M00033 has been validated on mouse in both WB and IF. Thus M00033 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-04-08
