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Product Info Summary
| SKU: | A01604S1177 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ICC, WB |
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Product info
Product Name
Anti-NOS3 (phospho-S1177) Antibody
SKU/Catalog Number
A01604S1177
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NOS3 (phospho-S1177) Antibody catalog # A01604S1177. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOS3 (phospho-S1177) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01604S1177)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Serine 1177.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01604S1177 is reactive to NOS3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
133275 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01604S1177 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-NOS3 (phospho-S1177) Antibody A01604S1177
Western blot (WB) analysis of p-NOS3 (S1177) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate
Lane2:sp2/0 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-NOS3 (phospho-S1177) Antibody A01604S1177
Western blot (WB) analysis of p-NOS3 (S1177) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:HEK293T treated with PMA (60ng/ml
Protein Target Info & Infographic
Gene/Protein Information For NOS3 (Source: Uniprot.org, NCBI)
Gene Name
NOS3
Full Name
Nitric oxide synthase, endothelial
Weight
133275 MW
Superfamily
NOS family
Alternative Names
Nitric oxide synthase, endothelial;1.14.13.39;Constitutive NOS;cNOS;EC-NOS;Endothelial NOS;eNOS;N NOS3 ECNOS, eNOS nitric oxide synthase 3 nitric oxide synthase, endothelial|EC-NOS|NOS type III|NOSIII|cNOS|constitutive NOS|endothelial NOS|nitric oxide synthase 3 (endothelial cell)|nitric oxide synthase 3 transcript variant eNOS-delta20|nitric oxide synthase 3 transcript variant eNOS-delta20-21|nitric oxide synthase 3 transcript variant eNOS-delta21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOS3, check out the NOS3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOS3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOS3 (phospho-S1177) Antibody (A01604S1177)
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