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Product Info Summary
| SKU: | A15072-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Nms Antibody
View all Neuromedin S Antibodies
SKU/Catalog Number
A15072-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Nms Antibody catalog # A15072-3. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Nms Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15072-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Nms recombinant protein (Position: D36-N144).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15072-3 is reactive to Nms in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
19908 MW
Background of Neuromedin S
Neuromedin S is a 36-amino acid neuropeptide found in the brain of humans and other mammals. This gene encodes a member of the neuromedin family of neuropeptides. The encoded protein is a precursor that is proteolytically processed to generate a biologically active neuropeptide that plays a role in the regulation of circadian rhythm, anorexigenic action, antidiuretic action, cardiovascular function and stimulation of oxytocin and vasopressin release. Mice lacking the encoded neuropeptide exhibit decreased heart rate without any accompanying changes in blood pressure. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature peptide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15072-3 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Nms using anti-Nms antibody (A15072-3).
Nms was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nms Antibody (A15072-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of Nms using anti-Nms antibody (A15072-3).
Nms was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nms Antibody (A15072-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nms (Source: Uniprot.org, NCBI)
Gene Name
Nms
Full Name
Neuromedin-S
Weight
19908 MW
Superfamily
NmU family
Alternative Names
Transmembrane protein 240;TMEM240;C1orf70; NMS neuromedin S neuromedin-S|neuromedin S precursor-related peptide/neuromedin S preproprotein|prepro-NMS
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nms, check out the Nms Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nms: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nms Antibody (A15072-3)
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