Product Info Summary
SKU: | PA1687 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-NLRP4G Antibody
SKU/Catalog Number
PA1687
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NLRP4G Antibody catalog # PA1687. Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NLRP4G Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1687)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NLRP4G.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1687 is reactive to Nlrp4g in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
85411 MW
Background of Nlrp4g
Stra8, stimulated by retinoic acid 8, is expressed in embryonic ovaries just before meiotic initiation, whereas in testis it is expressed after birth. The STRA8 gene contains 9 exons. And this gene is mapped to chromosome 7q31. Retinoic acid (RA) signaling was required for Stra8 expression, and therefore initiation of meiosis, in embryonic mouse ovaries. RA was sufficient to induce Stra8 expression in embryonic testis. Cyp26-mediated RA metabolism was responsible for delaying Stra8 expression in embryonic testis. Since RA signaling regulated Stra8 expression in both embryonic ovaries and adult testis, it was said this portion of the meiotic initiation pathway may be identical in both sexes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1687 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Ovary Tissue
IHC: human lung cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-NLRP4G antibody, PA1687, Western blotting
WB: Mouse Ovary Tissue Lysate
Click image to see more details
Figure 2. IHC analysis of NLRP4G using anti-NLRP4G antibody (PA1687).
NLRP4G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NLRP4G Antibody (PA1687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Nlrp4g (Source: Uniprot.org, NCBI)
Gene Name
Nlrp4g
Full Name
Weight
85411 MW
Alternative Names
NLR family, pyrin domain containing 4G ;Nlrp4g ;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nlrp4g, check out the Nlrp4g Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nlrp4g: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NLRP4G Antibody (PA1687)
Hello CJ!
PA1687 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and localization of Nlrp4g in mouse preimplantation embryo
Peng H, Zhang W, Xiao T, Zhang Y. Reprod Fertil Dev. 2014 Jun;26(5):758-68. Doi: 10.1071/Rd12409. Nlrp4G Is An Oocyte-Specific Gene But Is Not Required For Oocyte Maturation In The Mouse.
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Customer Q&As
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1 Customer Q&As for Anti-NLRP4G Antibody
Question
We are currently using anti-NLRP4G antibody PA1687 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-NLRP4G antibody (PA1687) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24