Anti-NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker) Monoclonal Antibody

Product Name

Anti-NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker) Monoclonal Antibody

View all NKX2.2 Antibodies

SKU/Catalog Number

M04740

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker) Monoclonal Antibody (Catalog # M04740). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat, Chicken.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-NKX2.2 (Neuroendocrine & Ewing's Sarcoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04740)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: rNX2/294

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human NKX2.2 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M04740 is reactive to NKX2-2 in Chicken, Human, Mouse, Rat

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

30133 MW

Background of NKX2.2

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M04740 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For NKX2-2 (Source: Uniprot.org, NCBI)

Alternative Names

Homeobox protein Nkx-2.2;Homeobox protein NK-2 homolog B;NKX2-2;NKX2.2, NKX2B; NKX2-2 NKX2.2, NKX2B NK2 homeobox 2 homeobox protein Nkx-2.2|NK2 transcription factor related, locus 2|NK2 transcription factor-like protein B|homeobox protein NK-2 homolog B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis