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Product Info Summary
| SKU: | A04740 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Homeobox protein Nkx-2.2 NKX2-2 Antibody
SKU/Catalog Number
A04740
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Homeobox protein Nkx-2.2 NKX2-2 Antibody (Catalog # A04740). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NKX2-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Homeobox protein Nkx-2.2 NKX2-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04740)
Host
Rabbit
Contents
NKX2-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2. The immunogen is located within amino acids 90 - 140 of NKX2-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.
Reactive Species
A04740 is reactive to NKX2-2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30133 MW
Background of NKX2.2
NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04740 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NKX2-2 in rat kidney tissue lysate with NKX2-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of NKX2-2 in human kidney tissue with NKX2-2 antibody at 2.5 μg/mL.
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Immunofluorescence of NKX2-2 in human kidney tissue with NKX2-2 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NKX2-2 (Source: Uniprot.org, NCBI)
Gene Name
NKX2-2
Full Name
Homeobox protein Nkx-2.2
Weight
30133 MW
Superfamily
NK-2 homeobox family
Alternative Names
NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B NKX2-2 NKX2.2, NKX2B NK2 homeobox 2 homeobox protein Nkx-2.2|NK2 transcription factor related, locus 2|NK2 transcription factor-like protein B|homeobox protein NK-2 homolog B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NKX2-2, check out the NKX2-2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NKX2-2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein Nkx-2.2 NKX2-2 Antibody (A04740)
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