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Product Info Summary
| SKU: | PA2169 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-NKCC1/SLC12A2 Antibody Picoband®
View all NKCC1/SLC12A2 Antibodies
SKU/Catalog Number
PA2169
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NKCC1/SLC12A2 Antibody catalog # PA2169. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NKCC1/SLC12A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2169)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2169 is reactive to SLC12A2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated molecular weight
131447 MW
Background of NKCC1/SLC12A2
Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2169 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human A549 whole cell
IHC: human colorectal adenocarcinoma tissue, mouse colon tissue, rat colon tissue
IF: human colon cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A2 antigen affinity purified polyclonal antibody (Catalog # PA2169) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A2 at approximately 200 kDa. The expected band size for SLC12A2 is at 131 kDa.
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Figure 2. IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169).
SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169).
SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169).
SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169).
SLC12A2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SLC12A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC12A2
Full Name
Solute carrier family 12 member 2
Weight
131447 MW
Superfamily
SLC12A transporter family
Alternative Names
Solute carrier family 12 member 2;Basolateral Na-K-Cl symporter;Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 1;SLC12A2;NKCC1; SLC12A2 BSC, BSC2, KILQS, NKCC1, PPP1R141 solute carrier family 12 member 2 solute carrier family 12 member 2|basolateral Na-K-Cl symporter|bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1|bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2|protein phosphatase 1, regulatory subunit 141|solute carrier family 12 (sodium/potassium/chloride transporter), member 2|solute carrier family 12 (sodium/potassium/chloride transporters), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC12A2, check out the SLC12A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC12A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NKCC1/SLC12A2 Antibody Picoband® (PA2169)
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No publications found for PA2169
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Customer Q&As
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5 Customer Q&As for Anti-NKCC1/SLC12A2 Antibody Picoband®
Question
Is this PA2169 anti-NKCC1/SLC12A2 antibody reactive to the isotypes of SLC12A2?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
The immunogen of PA2169 anti-NKCC1/SLC12A2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-21
Question
Does anti-NKCC1/SLC12A2 antibody PA2169 work on monkey WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Our lab technicians have not validated anti-NKCC1/SLC12A2 antibody PA2169 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NKCC1/SLC12A2 antibody PA2169 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-NKCC1/SLC12A2 antibody PA2169 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
P. Dhar
Verified customer
Asked: 2018-02-28
Answer
The anti-NKCC1/SLC12A2 antibody (PA2169) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-28
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-NKCC1/SLC12A2 antibody PA2169. Please let me know if you require anything else.
C. Wu
Verified customer
Asked: 2016-11-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-30
Question
My question regarding product PA2169, anti-NKCC1/SLC12A2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Zhang
Verified customer
Asked: 2014-11-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2169 anti-NKCC1/SLC12A2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-11-18
