Anti-FRA1/FOSL1 Antibody Picoband™

Product Name

Anti-FRA1/FOSL1 Antibody Picoband™

View all FRA-1/FOSL1 Antibodies

SKU/Catalog Number

A03927-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FRA1/FOSL1 Antibody Picoband™ catalog # A03927-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FRA1/FOSL1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03927-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human FRA1, which shares 75.7% and 71.8% amino acid (aa) sequence identity with mouse and rat RA1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03927-1 is reactive to FOSL1 in Human

Applications

A03927-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

Background of FRA-1/FOSL1

Fos-related antigen 1 (FRA1) is a protein that in humans is encoded by the FOSL1 gene. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of FRA1 using anti-FRA1 antibody (A03927-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified polyclonal antibody (Catalog # A03927-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 29KD. The expected band size for FRA1 is at 29KD.

Gene/Protein Information For FOSL1 (Source: Uniprot.org, NCBI)

Alternative Names

FOSL1; FOS-like antigen 1; FOS-like antigen-1; fos-related antigen 1; FRA; FRA1; FRA-1 FOSL1 FRA, FRA1, fra-1 FOS like 1, AP-1 transcription factor subunit fos-related antigen 1|FOS like 1, AP-1 trancription factor subunit|FOS-like antigen-1

For more info on FOSL1, check out the FOSL1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FOSL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the FOSL1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis