Product Info Summary
SKU: | A03927-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-FRA1/FOSL1 Antibody Picoband®
View all FRA-1/FOSL1 Antibodies
SKU/Catalog Number
A03927-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FRA1/FOSL1 Antibody Picoband® catalog # A03927-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FRA1/FOSL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03927-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FRA1, which shares 75.7% and 71.8% amino acid (aa) sequence identity with mouse and rat RA1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03927-1 is reactive to FOSL1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.413kDa
Background of FRA-1/FOSL1
Fos-related antigen 1 (FRA1) is a protein that in humans is encoded by the FOSL1 gene. The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03927-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Positive Control
WB: human A375 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FRA1 using anti-FRA1 antibody (A03927-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified polyclonal antibody (Catalog # A03927-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 29KD. The expected band size for FRA1 is at 29KD.
Protein Target Info & Infographic
Gene/Protein Information For FOSL1 (Source: Uniprot.org, NCBI)
Gene Name
FOSL1
Full Name
Fos-related antigen 1
Weight
29.413kDa
Superfamily
bZIP family
Alternative Names
Fos-related antigen 1; FRA-1; FOSL1; FRA1 FOSL1 FRA, FRA1, fra-1 FOS like 1, AP-1 transcription factor subunit fos-related 1|FOS like 1, AP-1 trancription factor subunit|FOS-like -1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOSL1, check out the FOSL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOSL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FRA1/FOSL1 Antibody Picoband® (A03927-1)
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Customer Q&As
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5 Customer Q&As for Anti-FRA1/FOSL1 Antibody Picoband®
Question
Would anti-FRA1/FOSL1 antibody A03927-1 work on bovine WB with blood?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Our lab technicians have not tested anti-FRA1/FOSL1 antibody A03927-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-FRA1/FOSL1 antibody A03927-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-24
Question
We are currently using anti-FRA1/FOSL1 antibody A03927-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
The anti-FRA1/FOSL1 antibody (A03927-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-27
Question
Would anti-FRA1/FOSL1 antibody A03927-1 work for WB with cervix carcinoma?
S. Yang
Verified customer
Asked: 2016-11-18
Answer
According to the expression profile of cervix carcinoma, FOSL1 is highly expressed in cervix carcinoma. So, it is likely that anti-FRA1/FOSL1 antibody A03927-1 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2016-11-18
Question
I see that the anti-FRA1/FOSL1 antibody A03927-1 works with WB, what is the protocol used to produce the result images on the product page?
L. Krishna
Verified customer
Asked: 2016-05-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-19
Question
Is there a BSA free version of anti-FRA1/FOSL1 antibody A03927-1 available?
S. Walker
Verified customer
Asked: 2014-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FRA1/FOSL1 antibody A03927-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-02-20