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Product Info Summary
| SKU: | M09715 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-NIT1 Mouse Monoclonal Antibody [Clone ID: OTI3A11]
View all Nitrilase 1 Antibodies
SKU/Catalog Number
M09715
Size
100 µl
Description
Boster Bio Anti-NIT1 mouse monoclonal antibody, clone OTI3A11 (formerly 3A11). Catalog# M09715. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-NIT1 Mouse Monoclonal Antibody [Clone ID: OTI3A11] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09715)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3A11
Isotype
IgG1
Immunogen
Full length human recombinant protein of human NIT1 (NP_005591) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09715 is reactive to NIT1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
35.896kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09715 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT1.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-NIT1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either pCMV6-ENTRY NIT1 (Red) or empty vector control plasmid (Blue) were immunostained with anti-NIT1 mouse monoclonal (M09715)
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Anti-NIT1 mouse monoclonal antibody (M09715) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT1.
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Immunofluorescent staining of HT29 cells using anti-NIT1 mouse monoclonal antibody (M09715).
Protein Target Info & Infographic
Gene/Protein Information For NIT1 (Source: Uniprot.org, NCBI)
Gene Name
NIT1
Full Name
Deaminated glutathione amidase
Weight
35.896kDa
Superfamily
carbon-nitrogen hydrolase superfamily
Alternative Names
EC 3.5; MGC57670; nitrilase 1; nitrilase homolog 1 NIT1 nitrilase 1 deaminated glutathione amidase|dGSH amidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NIT1, check out the NIT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NIT1 Mouse Monoclonal Antibody [Clone ID: OTI3A11] (M09715)
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No publications found for M09715
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