Anti-Nicastrin/NCSTN Antibody Picoband™

Product Name

Anti-Nicastrin/NCSTN Antibody Picoband™

View all Nicastrin Antibodies

SKU/Catalog Number

A03144-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-heavy chain Nicastrin/NCSTN Antibody Picoband™ catalog # A03144-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Nicastrin/NCSTN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03144-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Nicastrin/NCSTN recombinant protein (Position: N34-E669).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03144-1 is reactive to NCSTN in Human, Mouse, Rat

Applications

A03144-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

65411 MW

Background of Nicastrin

Nicastrin, also known asNCSTN, is aproteinthat is a part of the gamma secretaseprotein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretaseprotein complex, all of which undergo significant post-translational modificationbefore becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex.The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly.The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, anenzymeinvolved in the degradation of amyloid beta fragment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Nicastrin/NCSTN using anti-Nicastrin/NCSTN antibody (A03144-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat pancreas tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nicastrin/NCSTN antigen affinity purified polyclonal antibody (Catalog # A03144-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nicastrin/NCSTN at approximately 120 kDa. The expected band size for Nicastrin/NCSTN is at 120 kDa.

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Figure 2. IF analysis of Nicastrin/NCSTN using anti-Nicastrin/NCSTN antibody (A03144-1).
Nicastrin/NCSTN was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Nicastrin/NCSTN Antibody (A03144-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For NCSTN (Source: Uniprot.org, NCBI)

Alternative Names

anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253APH2; NCSTN; Nicastrin NCSTN ATAG1874 nicastrin nicastrin|anterior pharynx-defective 2

For more info on NCSTN, check out the NCSTN Infographic

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In this infographic, you will see the following information for NCSTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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