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Product Info Summary
| SKU: | M01187 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Ferret, Human, Monkey, Rabbit, Cat, Baboon |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody
View all NGFR/TNFRSF16/p75NTR Antibodies
SKU/Catalog Number
M01187
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody (Catalog # M01187). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Baboon, Cat, Rabbit, Ferret.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01187)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM299
Isotype
IgG1, kappa
Immunogen
NGFR from A875 melanoma cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01187 is reactive to NGFR in Ferret, Human, Monkey, Rabbit, Cat, Baboon
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
45183 MW
Background of NGFR/TNFRSF16/p75NTR
This monoclonal antibody recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01187 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-NGFR Monoclonal Antibody (SPM299).
Protein Target Info & Infographic
Gene/Protein Information For NGFR (Source: Uniprot.org, NCBI)
Gene Name
NGFR
Full Name
Tumor necrosis factor receptor superfamily member 16
Weight
45183 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 16;Gp80-LNGFR;Low affinity neurotrophin receptor p75NTR;Low-affinity nerve growth factor receptor;NGF receptor;p75 ICD;CD271;NGFR;TNFRSF16; NGFR CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR nerve growth factor receptor tumor necrosis factor receptor superfamily member 16|NGF receptor|TNFR superfamily, member 16|low affinity neurotrophin receptor p75NTR|low-affinity nerve growth factor receptor|p75 ICD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGFR, check out the NGFR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Monoclonal Antibody (M01187)
Hello CJ!
M01187 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The expressions of NGF and VEGF in the fracture tissues are closely associated with accelerated clavicle fracture healing in patients with traumatic brain injury
Derivation of corneal endothelial cell-like cells from rat neural crest cells in vitro
Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia
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