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Product Info Summary
| SKU: | A00284S529-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-NFkB p65 (Phospho-S529) RELA Antibody
SKU/Catalog Number
A00284S529-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NFkB p65 (Phospho-S529) RELA Antibody catalog # A00284S529-1. Tested in WB,IP,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB p65 (Phospho-S529) RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00284S529-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00284S529-1 is reactive to RELA in Human
Reconstitution
Calculated molecular weight
60219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00284S529-1 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
FC: 1:50-1:100
Validation Images & Assay Conditions
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HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes. NIH/3T3 cells were treated by TNF-α(20ng/mL) for 30 minutes.
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Flow cytometric analysis of Daudi cells with Phospho-NF-kB p65(S529) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For RELA (Source: Uniprot.org, NCBI)
Gene Name
RELA
Full Name
Transcription factor p65
Weight
60219 MW
Alternative Names
Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;RELA;NFKB3; RELA CMCU, NFKB3, p65 RELA proto-oncogene, NF-kB subunit transcription factor p65|NF-kappa-B p65delta3|NF-kappa-B transcription factor p65|nuclear factor NF-kappa-B p65 subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 3|v-rel avian reticuloendotheliosis viral oncogene homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RELA, check out the RELA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RELA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB p65 (Phospho-S529) RELA Antibody (A00284S529-1)
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