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Product Info Summary
| SKU: | A00283-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-NFkB-p105 (Ab-927) NFKB1 Antibody
SKU/Catalog Number
A00283-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (Catalog # A00283-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00283-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.925~929 (C-D-S-G-V ) derived from Human NFkB-p105.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00283-1 is reactive to NFKB1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
105356 MW
Background of NFKB1
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00283-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p105(Ab-927) Antibody #A00283-1.
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Figure 2. Western blot analysis of NFKB1 using anti-NFKB1 antibody (A00283-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody (Catalog # A00283-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NFKB1.
Protein Target Info & Infographic
Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)
Gene Name
NFKB1
Full Name
Nuclear factor NF-kappa-B p105 subunit
Weight
105356 MW
Alternative Names
Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;Nuclear factor NF-kappa-B p50 subunit;NFKB1; NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKB1, check out the NFKB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (A00283-1)
Hello CJ!
A00283-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
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