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Product Info Summary
| SKU: | M01228-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-NFkB p100 / p52 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01228-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-NFkB p100 / p52 Rabbit Monoclonal Antibody catalog # M01228-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB p100 / p52 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01228-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
18N03
Isotype
IgG
Immunogen
A synthesized peptide derived from human NFkB p100 / p52
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01228-1 is reactive to NFKB2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
110 kDa
Calculated molecular weight
75592 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01228-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Jurkat whole cell, human A549 whole cell, human CACO-2 whole cell, human U251 whole cell, rat heart tissue, rat PC-12 whole cell, mouse heart tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NF-κB p100/p52 using anti-NF-κB p100/p52 antibody (M01228-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-κB p100/p52 antigen affinity purified monoclonal antibody (Catalog # M01228-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-κB p100/p52 at approximately 110 kDa. The expected band size for NF-κB p100/p52 is at 97 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NFKB2 (Source: Uniprot.org, NCBI)
Gene Name
NFKB2
Full Name
Nuclear factor NF-kappa-B p100 subunit
Weight
75592 MW
Alternative Names
ATP-binding cassette sub-family G member 1;ATP-binding cassette transporter 8;White protein homolog;ABCG1;ABC8, WHT1; NFKB2 CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 nuclear factor kappa B subunit 2 nuclear factor NF-kappa-B p100 subunit|DNA-binding factor KBF2|NFKB, p52/p100 subunit|lymphocyte translocation chromosome 10 protein|nuclear factor Kappa-B, subunit 2|nuclear factor NF-kappa-B p52 subunit|nuclear factor of Kappa light chain gene enhancer in B cells 2|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)|oncogene Lyt-10|transcription factor NFKB2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKB2, check out the NFKB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB p100 / p52 Rabbit Monoclonal Antibody (M01228-1)
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