Click image to see more details
Product Info Summary
| SKU: | M00283 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00283
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody catalog # M00283. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00283)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ID-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-κB (p105/p50)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00283 is reactive to NFKB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50, 105 kDa
Calculated molecular weight
105356 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00283 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human RT4 whole cell, human A431 whole cell, human SiHa whole cell, human CACO-2 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse NIH/3T3 whole cell, mouse RAW264.7 whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NF-κB p105/p50 using anti-NF-κB p105/p50 antibody (M00283).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-κB p105/p50 antigen affinity purified monoclonal antibody (M00283) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-κB p105/p50 at approximately 50, 105 kDa. The expected band size for NF-κB p105/p50 is at 50, 105 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB p105/p50 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)
Gene Name
NFKB1
Full Name
Nuclear factor NF-kappa-B p105 subunit
Weight
105356 MW
Alternative Names
Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;Nuclear factor NF-kappa-B p50 subunit;NFKB1; NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKB1, check out the NFKB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody (M00283)
Hello CJ!
M00283 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu QL,Wu J.Effects of Txk‑mediated activation of NF‑κB signaling pathway on neurological deficit and oxidative stress after ischemia‑reperfusion in rats.Mol Med Rep.2021 Jul;24(1):524.doi:10.3892/mmr.2021.12163.Epub 2021 May 26.PMID:34036382.
Species: Rat
M00283 usage in article: APP:WB, SAMPLE:PERICRANIUM TISSUE, DILUTION:1:3000
Wang H,Qiu M,Li Y,Cheng D,Yang D,Yuan W.Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma.Biomed Pharmacother.2020 Jun;126:109866.doi:10.1016/j.biopha.2020.109866.Epub 2020 Feb 27.PMID:32114356.
Species: Mouse
M00283 usage in article: APP:IHC, SAMPLE:MELANOMA TUMOR TISSUE, DILUTION:NA
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-NF-Kappa B (p105/p50) NFKB1 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
