Product Info Summary
SKU: | M01006 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Neurokinin 1 Receptor Monoclonal Antibody
SKU/Catalog Number
M01006
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Neurokinin 1 Receptor Monoclonal Antibody catalog # M01006. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurokinin 1 Receptor Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01006)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFBH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Neurokinin 1 Receptor This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01006 is reactive to TACR1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
48 kDa
Calculated molecular weight
44740 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01006 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: human Raji whole cell, human K562 whole cell, human MOLT-4 whole cell, rat spleen tissue, mouse kidney tissue, mouse spleen tissue
IHC: human lung cancer tissue, mouse colon tissue, rat colon tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurokinin 1 Receptor antigen affinity purified monoclonal antibody (Catalog # M01006) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurokinin 1 Receptor at approximately 48 kDa. The expected band size for Neurokinin 1 Receptor is at 46 kDa.
Click image to see more details
Figure 2. IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006).
Neurokinin 1 Receptor was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006).
Neurokinin 1 Receptor was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Neurokinin 1 Receptor using anti-Neurokinin 1 Receptor antibody (M01006).
Neurokinin 1 Receptor was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Neurokinin 1 Receptor Antibody (M01006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TACR1 (Source: Uniprot.org, NCBI)
Gene Name
TACR1
Full Name
Substance-P receptor
Weight
44740 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
TAR DNA-binding protein 43;TDP-43;TARDBP;TDP43; TACR1 NK1R, NKIR, SPR, TAC1R tachykinin receptor 1 substance-P receptor|NK-1 receptor|NK-1R|tachykinin receptor 1 (substance P receptor; neurokinin-1 receptor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TACR1, check out the TACR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TACR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurokinin 1 Receptor Monoclonal Antibody (M01006)
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