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Product Info Summary
| SKU: | A00043 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Neurofibromin NF1 Antibody
View all Neurofibromin 1 Antibodies
SKU/Catalog Number
A00043
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Neurofibromin NF1 Antibody catalog # A00043. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neurofibromin NF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00043)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NF1. AA range:1551-1600
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00043 is reactive to NF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
319372 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00043 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HepG2 cells using Neurofibromin Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Neurofibromin Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NF1 (Source: Uniprot.org, NCBI)
Gene Name
NF1
Full Name
Neurofibromin
Weight
319372 MW
Alternative Names
Neurofibromin;Neurofibromatosis-related protein NF-1;Neurofibromin truncated;NF1; NF1 NFNS, VRNF, WSS neurofibromin 1 neurofibromin|neurofibromatosis 1|neurofibromatosis-related protein NF-1|truncated neurofibromin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NF1, check out the NF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurofibromin NF1 Antibody (A00043)
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No publications found for A00043
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Customer Q&As
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3 Customer Q&As for Anti-Neurofibromin NF1 Antibody
Question
I see that the anti-Neurofibromin antibody A00043 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-25
Question
Can you help my question with product A00043, anti-Neurofibromin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00043 anti-Neurofibromin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-14
Question
I was wanting to use your anti-Neurofibromin antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
S. Roberts
Verified customer
Asked: 2014-08-27
Answer
It shows on the product datasheet, A00043 anti-Neurofibromin antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-27
