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Product Info Summary
| SKU: | A06700-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Neurocan/NCAN Antibody Picoband®
SKU/Catalog Number
A06700-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neurocan/NCAN Antibody Picoband® catalog # A06700-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Neurocan/NCAN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06700-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Neurocan/NCAN recombinant protein (Position: H1140-A1179).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06700-2 is reactive to NCAN in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
142 kDa
Calculated molecular weight
104553 MW
Background of Neurocan
Neurocan core protein is a protein that in humans is encoded by the NCAN gene. Neurocan is a member of the lectican / chondroitin sulfate proteoglycan protein families and consists of neurocan core protein and chondroitin sulfate. It is thought to be involved in the modulation of cell adhesion and migration. In the human subjects, it was found that NCAN genotype was strongly associated with manic symptoms but not with depressive symptoms. In the mice, the absence of functional Neurocan resulted in a variety of manic-like behaviors, which could be normalized by administering lithium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06700-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Neurocan/NCAN using anti-Neurocan/NCAN antibody (A06700-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurocan/NCAN antigen affinity purified polyclonal antibody (Catalog # A06700-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurocan/NCAN at approximately 142 kDa. The expected band size for Neurocan/NCAN is at 136 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NCAN (Source: Uniprot.org, NCBI)
Gene Name
NCAN
Full Name
Neurocan core protein
Weight
104553 MW
Superfamily
aggrecan/versican proteoglycan family
Alternative Names
Neurocan core protein; Chondroitin sulfate proteoglycan 3; NCAN; CSPG3, NEUR; NCAN CSPG3 neurocan neurocan core protein|chondroitin sulfate proteoglycan 3 (neurocan)|neurocan proteoglycan
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCAN, check out the NCAN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neurocan/NCAN Antibody Picoband® (A06700-2)
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