Product Info Summary
SKU: | M00806 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC |
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Product info
Product Name
Anti-Nestin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00806
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nestin Rabbit Monoclonal Antibody catalog # M00806. Tested in IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nestin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FBH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nestin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00806 is reactive to NES in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
180 kDa
Calculated molecular weight
177439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00806 is guaranteed for IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human kidney tissue
ICC/IF: Hela cell, PC12 cell
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using Nestin Antibody.
Click image to see more details
Immunofluorescent analysis of Hela cells, using Nestin Antibody.
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Immunofluorescent analysis of PC12 cells, using Nestin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NES (Source: Uniprot.org, NCBI)
Gene Name
NES
Full Name
Nestin
Weight
177439 MW
Superfamily
intermediate filament family
Alternative Names
Nestin;NES;Nbla00170; NES Nbla00170 nestin nestin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NES, check out the NES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nestin Rabbit Monoclonal Antibody (M00806)
Hello CJ!
M00806 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng FW,Liu FS,Liu WH,Li L,Jie LL.Formation of new lymphatic vessels in glioma: An immunohistochemical analysis.Neuropathology.2020 Jun;40(3):215-223.doi:10.1111/neup.12625.Epub 2020 Jan 20.PMID:31960509;PMCID:PMC 7317190.
Species: Human
M00806 usage in article: APP:IHC, SAMPLE:GILOMA TISSUE, DILUTION:1:100
Isolation of neural crest%u2010derived stem cells from rat embryonic mandibular processes
Expression of nestin in the podocytes of normal and diseased human kidneys
Molecular and immunochemical demonstration of a novel member of Bf/C2 homolog in amphioxus Branchiostoma belcheri: implications for involvement of hepatic %u2026
Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats
Insulin-Producing Cells Derived from Human Embryonic Stem Cells: Comparison of Definitive Endoderm- and Nestin-Positive Progenitor-Based Differentiation Strategies
Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells
Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells
Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells
Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells
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Customer Q&As
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3 Customer Q&As for Anti-Nestin Rabbit Monoclonal Antibody
Question
We have observed staining in human liver. What should we do? Is anti-Nestin Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
According to literature liver does express NES. According to Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414
Boster Scientific Support
Answered: 2020-04-30
Question
I am interested in using your anti-Nestin Rabbit Monoclonal antibody for negative regulation of protein binding studies. Has this antibody been tested with western blotting on human melanoma tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We appreciate your inquiry. This M00806 anti-Nestin Rabbit Monoclonal antibody is tested on human melanoma tissue. It is guaranteed to work for IHC in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-29
Question
We are currently using anti-Nestin Rabbit Monoclonal antibody M00806 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
A. Williams
Verified customer
Asked: 2016-03-09
Answer
The anti-Nestin Rabbit Monoclonal antibody (M00806) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-09