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Product Info Summary
| SKU: | M00806-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nestin Monoclonal Antibody
SKU/Catalog Number
M00806-1
Size
100ul
Description
Boster Bio Anti-Nestin Monoclonal Antibody catalog # M00806-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nestin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00806-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Recombinant construct, amino acids 317-630 of the human protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00806-1 is reactive to NES in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
177439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00806-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000 IF/ICC: 1:500.
Validation Images & Assay Conditions
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Western blot analysis analysis of tissue and cell lysates with mouse mAB against nestin, M00806-1, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.
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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, M00806-1, dilution 1:500 in red, and costained with chicken pAb to MAP2,
Protein Target Info & Infographic
Gene/Protein Information For NES (Source: Uniprot.org, NCBI)
Gene Name
NES
Full Name
Nestin
Weight
177439 MW
Superfamily
intermediate filament family
Alternative Names
Nestin;NES;Nbla00170; NES Nbla00170 nestin nestin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NES, check out the NES Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NES: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nestin Monoclonal Antibody (M00806-1)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Nestin Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for placenta using anti-Nestin Monoclonal antibody M00806-1. Please let me know if you require anything else.
J. Anderson
Verified customer
Asked: 2020-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
Is this M00806-1 anti-Nestin Monoclonal antibody reactive to the isotypes of NES?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
The immunogen of M00806-1 anti-Nestin Monoclonal antibody is Recombinant construct, amino acids 317-630 of the human protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-17
Question
I was wanting to use to test anti-Nestin Monoclonal antibody M00806-1 on mouse placenta for research purposes, then I may be interested in using anti-Nestin Monoclonal antibody M00806-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-Nestin Monoclonal antibody M00806-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
We have been able to see staining in rat cervix carcinoma. What should we do? Is anti-Nestin Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
According to literature cervix carcinoma does express NES. According to Uniprot.org, NES is expressed in metanephric glomerulus, placenta, neuroblastoma, cervix carcinoma, liver, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have NES expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 18318008, 24275569
Neuroblastoma, Pubmed ID: 12880961
Placenta, Pubmed ID: 1478958, 16710414
Boster Scientific Support
Answered: 2019-12-31
Question
Does M00806-1 anti-Nestin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
As indicated on the product datasheet, M00806-1 anti-Nestin Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-19
Question
We ordered your anti-Nestin Monoclonal antibody for WB on metanephric glomerulus a few years ago. I am using mouse, and We want to use the antibody for IHC next. you antibody examining metanephric glomerulus as well as neuroblastoma in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
M. Roberts
Verified customer
Asked: 2019-10-11
Answer
I took a look at the website and datasheets of our anti-Nestin Monoclonal antibody and it appears that M00806-1 has been validated on mouse in both WB and IHC. Thus M00806-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-11
Question
Would anti-Nestin Monoclonal antibody M00806-1 work on zebrafish WB with metanephric glomerulus?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Our lab technicians have not validated anti-Nestin Monoclonal antibody M00806-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Nestin Monoclonal antibody M00806-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish metanephric glomerulus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
I have a question about product M00806-1, anti-Nestin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00806-1 anti-Nestin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-06
Question
We are currently using anti-Nestin Monoclonal antibody M00806-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The anti-Nestin Monoclonal antibody (M00806-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-15
Question
Does anti-Nestin Monoclonal antibody M00806-1 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
According to the expression profile of placenta, NES is highly expressed in placenta. So, it is likely that anti-Nestin Monoclonal antibody M00806-1 will work for IHC with placenta.
Boster Scientific Support
Answered: 2019-06-20
Question
Is a blocking peptide available for product anti-Nestin Monoclonal antibody (M00806-1)?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We do provide the blocking peptide for product anti-Nestin Monoclonal antibody (M00806-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-Nestin Monoclonal antibody M00806-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-02
Question
Is there a BSA free version of anti-Nestin Monoclonal antibody M00806-1 available?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Nestin Monoclonal antibody M00806-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-13
Question
I was wanting to use your anti-Nestin Monoclonal antibody for IHC for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
It shows on the product datasheet, M00806-1 anti-Nestin Monoclonal antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-25
Question
I am interested in using your anti-Nestin Monoclonal antibody for negative regulation of catalytic activity studies. Has this antibody been tested with western blotting on human neuroblastoma cells? We would like to see some validation images before ordering.
J. Collins
Verified customer
Asked: 2016-09-27
Answer
We appreciate your inquiry. This M00806-1 anti-Nestin Monoclonal antibody is validated on rat glioma cells, human neuroblastoma cells. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-09-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Nestin Monoclonal antibody M00806-1. Let me know if you need anything else.
L. Walker
Verified customer
Asked: 2015-02-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-17
