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Product Info Summary
| SKU: | M02192 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nesprin 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02192
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nesprin 1 Rabbit Monoclonal Antibody catalog # M02192. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nesprin 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02192)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEFE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nesprin 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02192 is reactive to SYNE1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
27 kDa
Calculated molecular weight
58951 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02192 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SYNE1 (Source: Uniprot.org, NCBI)
Gene Name
SYNE1
Full Name
Nesprin-1
Weight
58951 MW
Superfamily
nesprin family
Alternative Names
Caspase-10;CASP-10;3.4.22.63;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2;FLICE2;ICE-like apoptotic protease 4;Caspase-10 subunit p23/17;Caspase-10 subunit p12;CASP10;MCH4; SYNE1 8B, AMC3, AMCM, ARCA1, C6orf98, CPG2, EDMD4, KASH1, MYNE1, Nesp1, SCAR8, dJ45H2.2 spectrin repeat containing nuclear envelope protein 1 nesprin-1|CPG2 full length|KASH domain-containing protein 1|enaptin|myocyte nuclear envelope protein 1|nesprin 1|spectrin repeat containing, nuclear envelope 1|synaptic nuclear envelope protein 1|synaptic nuclei expressed gene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYNE1, check out the SYNE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nesprin 1 Rabbit Monoclonal Antibody (M02192)
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No publications found for M02192
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Nesprin 1 Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02192?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-Nesprin 1 Monoclonal antibody M02192. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
I see that the anti-Nesprin 1 Monoclonal antibody M02192 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-23
Question
My question regards to test anti-Nesprin 1 Monoclonal antibody M02192 on rat liver for research purposes, then I may be interested in using anti-Nesprin 1 Monoclonal antibody M02192 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The products we sell, including anti-Nesprin 1 Monoclonal antibody M02192, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-14
Question
Is a blocking peptide available for product anti-Nesprin 1 Monoclonal antibody (M02192)?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
We do provide the blocking peptide for product anti-Nesprin 1 Monoclonal antibody (M02192). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-12
Question
Will M02192 anti-Nesprin 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Jones
Verified customer
Asked: 2017-02-09
Answer
As indicated on the product datasheet, M02192 anti-Nesprin 1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-09
Question
We are currently using anti-Nesprin 1 Monoclonal antibody M02192 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
T. Banerjee
Verified customer
Asked: 2015-04-30
Answer
The anti-Nesprin 1 Monoclonal antibody (M02192) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-30
