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Product Info Summary
| SKU: | M00547 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NEDD8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00547
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NEDD8 Rabbit Monoclonal Antibody catalog # M00547. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEDD8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00547)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOAE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NEDD8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00547 is reactive to NEDD8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa, 48 kDa
Calculated molecular weight
9072 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00547 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of NEDD8 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NEDD8 (Source: Uniprot.org, NCBI)
Gene Name
NEDD8
Full Name
NEDD8
Weight
9072 MW
Superfamily
ubiquitin family
Alternative Names
NEDD8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8;NEDD-8;Ubiquitin-like protein Nedd8;NEDD8; NEDD8 NEDD-8 NEDD8 ubiquitin like modifier NEDD8|neddylin|neural precursor cell expressed, developmentally down-regulated 8|ubiquitin-like protein Nedd8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEDD8, check out the NEDD8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEDD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEDD8 Rabbit Monoclonal Antibody (M00547)
Hello CJ!
M00547 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang B,Adams Z,Moonah S,Shi H,Maupin-Furlow J,Moskovitz J.The Antioxidant Enzyme Methionine Sulfoxide Reductase A (MsrA) Interacts with Jab1/CSN5 and Regulates Its Function.Antioxidants (Basel).2020 May 24;9(5):452.doi:10.3390/antiox9050452.PMID:32456285
Species: Mouse
M00547 usage in article: APP:WB, SAMPLE:BRAIN AND LIVER EXTRACTS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-NEDD8 Rabbit Monoclonal Antibody
Question
Would anti-NEDD8 Rabbit Monoclonal antibody M00547 work on dog IF with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
Our lab technicians have not tested anti-NEDD8 Rabbit Monoclonal antibody M00547 on dog. You can run a BLAST between dog and the immunogen sequence of anti-NEDD8 Rabbit Monoclonal antibody M00547 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog anterior cingulate cortex in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
See below the WB image, lot number and protocol we used for fibrosarcoma using anti-NEDD8 Rabbit Monoclonal antibody M00547. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-15
Question
Do you have a BSA free version of anti-NEDD8 Rabbit Monoclonal antibody M00547 available?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NEDD8 Rabbit Monoclonal antibody M00547 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-01
Question
I see that the anti-NEDD8 Rabbit Monoclonal antibody M00547 works with WB, what is the protocol used to produce the result images on the product page?
E. Yang
Verified customer
Asked: 2016-01-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-01-28
Question
Is a blocking peptide available for product anti-NEDD8 Rabbit Monoclonal antibody (M00547)?
A. Miller
Verified customer
Asked: 2015-12-03
Answer
We do provide the blocking peptide for product anti-NEDD8 Rabbit Monoclonal antibody (M00547). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-12-03
