Anti-NEDD8 Rabbit Monoclonal Antibody

Product Name

Anti-NEDD8 Rabbit Monoclonal Antibody

View all NEDD8 Antibodies

SKU/Catalog Number

M00547

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NEDD8 Rabbit Monoclonal Antibody catalog # M00547. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEDD8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00547)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOAE-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NEDD8

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00547 is reactive to NEDD8 in Human, Mouse, Rat

Applications

M00547 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa, 48 kDa

Calculated molecular weight

9072 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20

Validation Images & Assay Conditions

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Western blot analysis of NEDD8 expression in K562 cell lysate.

Gene/Protein Information For NEDD8 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8 NEDD8 NEDD-8 NEDD8 ubiquitin like modifier NEDD8|neddylin|neural precursor cell expressed, developmentally down-regulated 8|ubiquitin-like protein Nedd8

For more info on NEDD8, check out the NEDD8 Infographic

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In this infographic, you will see the following information for NEDD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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