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Product Info Summary
| SKU: | MA1069 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Nebulin Antibody (Monoclonal, NB2)
SKU/Catalog Number
MA1069
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Nebulin Antibody (Monoclonal, NB2) catalog # MA1069. Tested in WB applications. This antibody reacts with Chicken.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nebulin Antibody (Monoclonal, NB2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
NB2
Isotype
Mouse IgG1
Immunogen
Chicken breast nebulin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1069 is reactive to NEB in Chicken
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
773 kDa
Calculated molecular weight
772914 MW
Background of Nebulin
Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1069 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1-2 μg/ml, Chicken
Positive Control
WB: Mouse Skeletal Muscle Tissue
Validation Images & Assay Conditions
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Anti-Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD
Protein Target Info & Infographic
Gene/Protein Information For NEB (Source: Uniprot.org, NCBI)
Gene Name
NEB
Full Name
Nebulin
Weight
772914 MW
Alternative Names
Nebulin;NEB; NEB NEB177D, NEM2 nebulin nebulin|nemaline myopathy type 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEB, check out the NEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nebulin Antibody (Monoclonal, NB2) (MA1069)
Hello CJ!
MA1069 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.
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Customer Q&As
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2 Customer Q&As for Anti-Nebulin Antibody (Monoclonal, NB2)
Question
We are currently using anti-Nebulin antibody (Monoclonal, NB2) MA1069 for chicken tissue, and we are content with the WB results. The species of reactivity given in the datasheet says chicken. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Nebulin antibody (Monoclonal, NB2) (MA1069) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
We are interested in using your anti-Nebulin antibody (Monoclonal, NB2) for regulation of actin filament length studies. Has this antibody been tested with western blotting on mouse skeletal muscle tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
We appreciate your inquiry. This MA1069 anti-Nebulin antibody (Monoclonal, NB2) is validated on mouse skeletal muscle tissue, tissue lysate. It is guaranteed to work for WB in chicken. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-06
