Nebulin Antibodies

Nebulin (NEB)

This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system connected with the myofibrils. Binds and stabilize F-actin.

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Gene Information

Human
Gene Name:	NEB
Uniprot:	P20929
Entrez:	4703

Family

Belongs to:
No superfamily

Alternative Names

DKFZp686C1456; FLJ11505; FLJ36536; FLJ39568; FLJ39584; NEB177D; nebulin; NEM2

Molecular Weight

Mass (kDA):
772.914 kDA

Genomic Context

Human
Location:	2q23.3
Sequence:	2; NC_000002.12 (151485334..151734487, complement)

Tissue Specificity

Muscle specific. Located in the thin filament of striated muscle.

Subcellular Localizations

Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.

Diseases

• Myopathy
• Hypoxia
• Myopathies, Nemaline
• Hyperplasia
• Weakness
• Lung Diseases
• Neoplasms
• Anoxia
• Congenital Myopathy (disorder)
• Nervousness
• Carcinoma, Neuroendocrine
• Asthma
• Muscle Weakness

• Hypertensive Disease
• Lung Diseases, Obstructive
• Malignant Neoplasm Of Lung
• Pulmonary Hypertension
• Mastitis
• Carcinoma
• Fibrosis

Interacting Proteins

• ACO1
• DOCK9
• DYSF
• KLHL41
• MYPN

• SOS1
• SVIL
• TTN

Pathways

• Innervation
• Mitosis
• Lactation
• Cell Cycle
• Localization
• Exocytosis
• Lung Development
• Anaphase
• Pathogenesis
• Methylation
• Secretion
• Interphase
• Prophase

• Metaphase
• Ovulation
• Transport
• Fertilization
• Immune Response
• Parturition
• Spindle Assembly

PTMs

• Phosphorylation
• Oxidation
• Cleavage
• Nitration

• Amidation
• Decarboxylation
• Methylation
• Geranylgeranylation

Research Areas

• Cell Biology
• Cellular Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NEB

Best Uses of the NEB Marker in Boster Bio

Are you curious to learn more about BosterBio This article will provide information about the NEB-5alpha and SHuffle(r), markers, and how you can optimize your experiments. In addition to reading about the different types of NEB markers, you'll discover what the best use of each one is, and how to optimize your experiment with Boster Bio. You'll learn why these markers are so important for your research, and how you can optimize them for optimal results.

Escherichia coli NEB 5-alpha

The NEB-5 beta gene is a versatile genetic marker for Escherichia coli that improves phosphate soluble in bacterial cells. Its cloning and conversion efficiencies make it an ideal choice for routine cloning, subcloning, and electrocompetent recombination.

This gene is responsible DH5I+- selective fluoroquinolone resistance. The genome of the strain has not been published. However, the NEB 5alpha marker is now complete and will be an important reference to researchers working with this particular strain. Escherichia coli DH5I+ carries the recA gene, which confers resistance to fluoroquinolones.

Escherichia coli SHuffle(r)

The NEB gene in Escherichiacoli encodes the presence and type of a bacteriophage. This gene can be found within a phagemid made by GE Biosciences. It is expressed in phage PCANTAB5E. This phagemid contains tryptone 16g/L yeast 10g/L sodium 5mM glucose 2% ampicillin 100 ug/mL and 15%ethanol

109 bacteria cells were infected with M13K07MOI 30 from NEB Frankfurt, Germany. The phages were first cultured in 200mL 2xTYG–A. They were then removed from bacteria and kept at 4°C overnight. The titers of infected cells were used to calculate the phage input titers. They were called colony forming units (CFU).

PCR-based fusion primes were used to amplify pgsA–mCherry plasmid fragments using a P/F/P–EGFP R primer, and an S/EGFP R/S–EGFP primer pair. The PCR products were then used to clone the pLP–pgsA’ vector, which included the alr gene.

Boster Bio optimization

Flow procedures in Boster Bio are extensive, and optimizing your experiments can be a tricky process. There are many options available, so it can be difficult to choose the right procedure. These tips and guidelines will help you to make the most of the process. Boster Bio optimization tips will provide more information. These guides will help you optimize your experiments in a variety of ways. Using these guides can make your experiment as efficient as possible, and answer any questions you might have along the way.