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Product Info Summary
| SKU: | A12826 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-NDUFB1 Antibody
SKU/Catalog Number
A12826
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NDUFB1 Antibody (Catalog # A12826). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NDUFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12826)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NDUFB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12826 is reactive to NDUFB1 in Human
Reconstitution
Calculated molecular weight
6961 MW
Background of NDUFB1
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Triepels R.H.,
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Mao M., Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12826 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using NDUFB1 antibody A12826.
Protein Target Info & Infographic
Gene/Protein Information For NDUFB1 (Source: Uniprot.org, NCBI)
Gene Name
NDUFB1
Full Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
Weight
6961 MW
Superfamily
complex I NDUFB1 subunit family
Alternative Names
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1;Complex I-MNLL;CI-MNLL;NADH-ubiquinone oxidoreductase MNLL subunit;NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, N-terminally processed;NDUFB1; NDUFB1 CI-MNLL, CI-SGDH, MNLL NADH:ubiquinone oxidoreductase subunit B1 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1|NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa|NADH-ubiquinone oxidoreductase MNLL subunit|complex I MNLL subunit|complex I-MNLL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFB1, check out the NDUFB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFB1 Antibody (A12826)
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No publications found for A12826
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Customer Q&As
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5 Customer Q&As for Anti-NDUFB1 Antibody
Question
I have a question about product A12826, anti-NDUFB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12826 anti-NDUFB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-NDUFB1 antibody A12826 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-NDUFB1 antibody (A12826) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Is a blocking peptide available for product anti-NDUFB1 antibody (A12826)?
G. Kulkarni
Verified customer
Asked: 2019-06-03
Answer
We do provide the blocking peptide for product anti-NDUFB1 antibody (A12826). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-NDUFB1 antibody A12826 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-25
Question
We are interested in to test anti-NDUFB1 antibody A12826 on human corpus callosum for research purposes, then I may be interested in using anti-NDUFB1 antibody A12826 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Carter
Verified customer
Asked: 2015-04-15
Answer
The products we sell, including anti-NDUFB1 antibody A12826, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-15
