Product Info Summary
SKU: | M05876-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NDP52/CALCOCO2 Antibody Picoband™ (monoclonal, 9E2F2)
SKU/Catalog Number
M05876-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NDP52/CALCOCO2 Antibody Picoband™ (monoclonal, 9E2F2) catalog # M05876-2. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NDP52/CALCOCO2 Antibody Picoband™ (monoclonal, 9E2F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05876-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
9E2F2
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human NDP52/CALCOCO2 recombinant protein (Position: M1-L446).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M05876-2 is reactive to CALCOCO2 in Human, Mouse, Rat
Applications
M05876-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
Background of NDP52
This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
![m05876 2 calcoco2 primary antibodies wb testing 1 m05876 2 calcoco2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m05876-2-calcoco2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (M05876-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NDP52/CALCOCO2 antigen affinity purified monoclonal antibody (Catalog # M05876-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52 kDa. The expected band size for NDP52/CALCOCO2 is at 52 kDa.
![m05876 2 calcoco2 primary antibodies if testing 2 m05876 2 calcoco2 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m05876-2-calcoco2-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (M05876-2).
NDP52/CALCOCO2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-NDP52/CALCOCO2 Antibody (M05876-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![m05876 2 calcoco2 primary antibodies fcm testing 3 m05876 2 calcoco2 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m05876-2-calcoco2-primary-antibodies-fcm-testing-3.jpg)
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Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-NDP52/CALCOCO2 antibody (M05876-2).
Overlay histogram showing MCF-7 cells stained with M05876-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-NDP52/CALCOCO2 Antibody (M05876-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CALCOCO2 (Source: Uniprot.org, NCBI)
Gene Name
CALCOCO2
Full Name
Calcium-binding and coiled-coil domain-containing protein 2
Weight
Superfamily
CALCOCO family
Alternative Names
Antigen nuclear dot 52 kDa protein; calcium binding and coiled-coil domain 2; calcium-binding and coiled-coil domain-containing protein 2; NDP52MGC17318; Nuclear domain 10 protein 52; Nuclear domain 10 protein NDP52; Nuclear dot protein 52 CALCOCO2 NDP52 calcium binding and coiled-coil domain 2 calcium-binding and coiled-coil domain-containing protein 2|antigen nuclear dot 52 kDa protein|nuclear domain 10 protein 52|nuclear domain 10 protein NDP52|nuclear dot protein 52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALCOCO2, check out the CALCOCO2 Infographic
![CALCOCO2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALCOCO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDP52/CALCOCO2 Antibody Picoband™ (monoclonal, 9E2F2) (M05876-2)
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