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Product Info Summary
| SKU: | A04180-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ND4/Mtnd4 Antibody
SKU/Catalog Number
A04180-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ND4/Mtnd4 Antibody catalog # A04180-2. Tested in WB applications. This antibody reacts with Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ND4/Mtnd4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04180-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat ND4/Mtnd4, which shares 82.3% amino acid (aa) sequence identity with mouse ND4/Mtnd4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04180-2 is reactive to Mtnd4 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
51.882kDa
Background of Mtnd4
NADH-ubiquinone oxidoreductase chain 4 is a protein that in humans is encoded by the mitochondrial gene MT-ND4. The ND4 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variations in the MT-ND4 gene are associated with age-related macular degeneration (AMD), Leber's hereditary optic neuropathy (LHON), mesial temporal lobe epilepsy (MTLE) and cystic fibrosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04180-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Positive Control
WB: rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Mtnd4 using anti-Mtnd4 antibody (A04180-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mtnd4 antigen affinity purified polyclonal antibody (Catalog # A04180-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mtnd4 at approximately 48KD. The expected band size for Mtnd4 is at 52KD.
Protein Target Info & Infographic
Gene/Protein Information For Mtnd4 (Source: Uniprot.org, NCBI)
Gene Name
Mtnd4
Full Name
NADH-ubiquinone oxidoreductase chain 4
Weight
51.882kDa
Superfamily
complex I subunit 4 family
Alternative Names
NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; Mtnd4; mt-Nd4; Nd4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mtnd4, check out the Mtnd4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mtnd4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ND4/Mtnd4 Antibody (A04180-2)
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