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Product Info Summary
| SKU: | A01516-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-NCR1 Antibody
View all NKp46/NCR1 Antibodies
SKU/Catalog Number
A01516-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NCR1 Antibody catalog # A01516-2. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NCR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01516-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NCR1 recombinant protein (Position: A55-R258).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01516-2 is reactive to NCR1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
34.481kDa
Background of NKp46/NCR1
Natural cytotoxicity triggering receptor 1 is a protein that in humans is encoded by the NCR1 gene. Predicted to be involved in cellular defense response; regulation of natural killer cell mediated cytotoxicity; and signal transduction. Predicted to act upstream of or within defense response to virus and detection of virus. Predicted to be located in cell surface. Predicted to be part of SWI/SNF complex. Predicted to be active in plasma membrane. Biomarker of acquired immunodeficiency syndrome; anogenital venereal wart; hepatitis C; and lymphoproliferative syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01516-2 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: human tonsil tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of NCR1 using anti-NCR1 antibody (A01516-2).
NCR1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCR1 Antibody (A01516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of NCR1 using anti-NCR1 antibody (A01516-2).
NCR1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCR1 Antibody (A01516-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NCR1 (Source: Uniprot.org, NCBI)
Gene Name
NCR1
Full Name
Natural cytotoxicity triggering receptor 1
Weight
34.481kDa
Superfamily
natural cytotoxicity receptor (NCR) family
Alternative Names
Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; NKp46; hNKp46; CD335; NCR1; LY94 NCR1 CD335, LY94, NK-p46, NKP46 natural cytotoxicity triggering receptor 1 natural cytotoxicity triggering receptor 1|NK cell-activating receptor|lymphocyte 94 homolog (activating NK-receptor; NK-p46)|natural killer cell p46-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCR1, check out the NCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NCR1 Antibody (A01516-2)
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