Click image to see more details
Product Info Summary
| SKU: | M04036-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Nck2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04036-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Nck2 Rabbit Monoclonal Antibody catalog # M04036-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nck2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04036-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28N61
Isotype
IgG
Immunogen
A synthesized peptide derived from human Nck2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04036-1 is reactive to NCK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
35213 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04036-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human SH-SY5Y whole cell, human THP-1 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NCK2 using anti-NCK2 antibody (M04036-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCK2 antigen affinity purified monoclonal antibody (Catalog # M04036-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCK2 at approximately 43 kDa. The expected band size for NCK2 is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NCK2 (Source: Uniprot.org, NCBI)
Gene Name
NCK2
Full Name
Cytoplasmic protein NCK2
Weight
35213 MW
Alternative Names
Lymphatic vessel endothelial hyaluronic acid receptor 1;LYVE-1;Cell surface retention sequence-binding protein 1;CRSBP-1;Extracellular link domain-containing protein 1;Hyaluronic acid receptor;LYVE1;CRSBP1, HAR, XLKD1;UNQ230/PRO263; NCK2 GRB4, NCKbeta NCK adaptor protein 2 cytoplasmic protein NCK2|SH2/SH3 adaptor protein NCK-beta|growth factor receptor-bound protein 4|noncatalytic region of tyrosine kinase, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCK2, check out the NCK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nck2 Rabbit Monoclonal Antibody (M04036-1)
Hello CJ!
No publications found for M04036-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Nck2 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Nck2 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
