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Product Info Summary
| SKU: | A07424-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-NCBP1 Antibody
SKU/Catalog Number
A07424-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NCBP1 Antibody (Catalog # A07424-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NCBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NCBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07424-1)
Host
Rabbit
Contents
NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1. The immunogen is located within amino acids 420 - 470 of NCBP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
Reactive Species
A07424-1 is reactive to NCBP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
91839 MW
Background of NCBP1
NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07424-1 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NCBP1 in HeLa cell lysate with NCBP1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of NCBP1 in HeLa cells with NCBP1 antibody at 2.5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For NCBP1 (Source: Uniprot.org, NCBI)
Gene Name
NCBP1
Full Name
Nuclear cap-binding protein subunit 1
Weight
91839 MW
Superfamily
NCBP1 family
Alternative Names
NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein NCBP1 CBP80, NCBP, Sto1 nuclear cap binding protein subunit 1 nuclear cap-binding protein subunit 1|80 kDa nuclear cap-binding protein|NCBP 80 kDa subunit|nuclear cap binding protein subunit 1, 80kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCBP1, check out the NCBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NCBP1 Antibody (A07424-1)
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