NAT1 Antibodies

Arylamine N-acetyltransferase 1 (NAT1)

Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of different arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.

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Gene Information

Human
Gene Name:	NAT1
Uniprot:	P18440
Entrez:	9

Family

Belongs to:
arylamine N-acetyltransferase family

Alternative Names

AAC1N-acetyltransferase type 1; Arylamide acetylase 1; arylamine N-acetyltransferase 1; EC 2.3.1.5; MNAT; Monomorphic arylamine N-acetyltransferase; N-acetyltransferase 1 (arylamine N-acetyltransferase); NAT-1; NATI

Molecular Weight

Mass (kDA):
33.899 kDA

Genomic Context

Human
Location:	8p22
Sequence:	8; NC_000008.11 (18170419..18223689)

Subcellular Localizations

Cytoplasm.

Diseases

• Malignant Neoplasms
• Neoplasms
• Malignant Neoplasm Of Urinary Bladder
• Slow Acetylator Due To N-acetyltransferase Enzyme Variant
• Colorectal Cancer
• Bladder Neoplasm
• Carcinogenesis
• Malignant Neoplasm Of Breast
• Colorectal Neoplasms
• Mammary Neoplasms
• Carcinoma
• Salmonella Infections

• Malignant Neoplasm Of Prostate
• Malignant Neoplasm Of Lung
• Adenocarcinoma
• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Leukemia
• Malignant Tumor Of Colon
• Dna Adduction

Pathways

• Translation
• Conjugation
• Mating
• Dna Repair
• Localization
• Pathogenesis
• Sensitization
• Cell Cycle
• Transport
• Proteolysis
• Hypersensitivity
• Methylation
• Cell Proliferation

• Dna Replication
• Cell Death
• Cell Growth
• Spermatogenesis
• Cell Division
• Cell Differentiation
• Tube Development

PTMs

• Acetylation
• Hydroxylation
• Deamination
• Cleavage
• Methylation
• Reduction

• Deacetylation
• Oxidation
• Ubiquitination
• Demethylation
• Phosphorylation
• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NAT1

Boster Bio Review: Best Uses Of The NAT1 Marker

You've found the right place if you're looking for a reliable human marker of NAT1 protein. In this Boster Bio review, we'll discuss the NAT1 protein, the Primary-Secondary-ABC System, and the NAT1 Marker. This protein is an internationally applicable marker that can also be used by scientists from all over the globe.

NAT1Marker

Patients with metastatic breast carcinoma are more likely to survive if their NAT1 expression levels increase. However, there is a weak correlation between NAT1 and survival. The Gehan-Breslow-Wilcoxon statistic puts more weight on early events in overall survival. This study included 541 patients with no hormone therapy. Two groups were created for patients: high-expression and lower-expression. The high-expression groups had a greater overall survival rate, and a shorter period of time before the disease progressed.

The NAT1 gene could play a role for cell survival and proliferation. The expression of several genes in breast carcinoma was associated with NAT1 gene mRNA. These genes are associated also with different outcomes. Patients with higher levels of NAT1 expression had a better chance of survival than patients with lower levels. Patients with breast tumors that express ER+ estrogen receptors had a significantly longer survival rate than patients who had low levels of NAT1 expression. This suggests that high NAT1 expression could be a prognostic indicator in breast cancer patients who respond to chemotherapy.

The NAT1 gene expression was also associated with survival in the first five years of chemotherapy patients. In contrast, there was no correlation with NAT1 expression and survival among other groups. This is because low levels of NAT1 are associated with chemo-resistance, which increases the risk of relapse. Similarly, low levels of NAT1 mRNA in cancer patients are highly sensitive in identifying non-responders to chemotherapies.

The NAT1 genetic mutation has been linked to cancer. It is involved with the metabolism and production of aromatic amines. It is genetically polymorphic and can be found on chromosome8. It has been shown to be associated with cancer cell invasion and growth. This gene is crucial for drug treatment research and cancer research. There are many uses of NAT1.

Primary-Secondary-ABC System

The Primary-Secondary-ABCC System in Boster Bio enables researchers to efficiently and accurately detect protein targets. The Primary-Secondary-ABC System is based on the use of avidins conjugated to signal molecules, typically Horseradish Peroxide, HRP. This system has been demonstrated to be extremely sensitive, specific, and allows researchers easy identification of the protein of particular interest in samples. Other detection systems based upon organic polymers and/or polysaccharides are also available, such as Boster Bio’s Super Vision detector kits.

This method uses preformed avidin-biotin-peroxidase complexes as detection layers. These complexes contain four biotin-binding sites, allowing the detecting layer only to consist of one layer. The secondary antibody is biotin-labeled and attached to the avidin molecules to form a large complex that is equal to the primary antibody.

Human NAT1 protein

The NAT1 Protein is expressed in E.coli. For long-term storage, NAT1 should be stored at -20 or -80 degrees Celsius. It is important to avoid repeated freeze-thaw cycles, and to store the protein at a temperature that doesn't cause excessive temperature fluctuations. The buffer should contain 20 mM TrisHCl (20 mM NaCl), and 10% glycerol. This product can be used by scientists and researchers all over the world.