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Product Info Summary
| SKU: | A10553-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NAPRT Antibody Picoband®
SKU/Catalog Number
A10553-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NAPRT Antibody Picoband® catalog # A10553-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NAPRT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10553-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NAPRT recombinant protein (Position: A69-Q480).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10553-1 is reactive to NAPRT in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
10201 MW
Background of NAPRT
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10553-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NAPRT using anti-NAPRT antibody (A10553-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAPRT antigen affinity purified polyclonal antibody (Catalog # A10553-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAPRT at approximately 58 kDa. The expected band size for NAPRT is at 58 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NAPRT (Source: Uniprot.org, NCBI)
Gene Name
NAPRT
Full Name
Nicotinate phosphoribosyltransferase
Weight
10201 MW
Superfamily
NAPRTase family
Alternative Names
Neutrophil defensin 1;Defensin, alpha 1;HNP-1;HP-1;HP1;HP 1-56;Neutrophil defensin 2;HNP-2;HP-2;HP2;DEFA1;DEF1, DEFA2, MRS;DEFA1B; NAPRT NAPRT1, PP3856 nicotinate phosphoribosyltransferase nicotinate phosphoribosyltransferase|FHA-HIT-interacting protein|NAPRTase|nicotinate phosphoribosyltransferase domain containing 1|nicotinate phosphoribosyltransferase domain-containing protein 1|nicotinic acid phosphoribosyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAPRT, check out the NAPRT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAPRT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAPRT Antibody Picoband® (A10553-1)
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