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Product Info Summary
| SKU: | A09675 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-NADE BEX3 Antibody
View all NGFRAP1/BEX3/NADE Antibodies
SKU/Catalog Number
A09675
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NADE BEX3 Antibody (Catalog # A09675). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
NADE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NADE BEX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09675)
Host
Rabbit
Contents
NADE Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Reactive Species
A09675 is reactive to NGFRAP1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
12959 MW
Background of NGFRAP1/BEX3/NADE
The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09675 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NADE antibody can be used for detection of NADE by Western blot at 1 μg/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 μg/mL in the presence (A) or absence (B) of blocking peptide.
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Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For NGFRAP1 (Source: Uniprot.org, NCBI)
Gene Name
NGFRAP1
Full Name
Protein BEX3
Weight
12959 MW
Superfamily
BEX family
Alternative Names
Bex, BEX3, NADE, HGR74, DXS6984E, Protein BEX3, Brain-expressed X-linked protein 3 BEX3 Bex, DXS6984E, HGR74, NADE, NGFRAP1 brain expressed X-linked 3 protein BEX3|brain-expressed X-linked protein 3|nerve growth factor receptor (TNFRSF16) associated protein 1|ovarian granulosa cell 13.0 kDa protein HGR74|p75NTR-associated cell death executor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGFRAP1, check out the NGFRAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGFRAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NADE BEX3 Antibody (A09675)
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