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Product Info Summary
| SKU: | A08675 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NAC-1 NACC1 Monoclonal Antibody
SKU/Catalog Number
A08675
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NAC-1 NACC1 Monoclonal Antibody catalog # A08675. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NAC-1 NACC1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08675)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
6H2
Isotype
IgG
Immunogen
Purified recombinant fragment of human NAC-1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08675 is reactive to NACC1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
57258 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08675 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using NAC-1 Monoclonal antibody against HEK293 (1) and NACC1-hIgGFc transfected HEK293 (2) cell lysate.
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Immunohistochemistry (IHC) analysis of paraffin-embedded mammary cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using NAC-1 Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NACC1 (Source: Uniprot.org, NCBI)
Gene Name
NACC1
Full Name
Nucleus accumbens-associated protein 1
Weight
57258 MW
Alternative Names
Nucleus accumbens-associated protein 1;NAC-1;BTB/POZ domain-containing protein 14B;NACC1;BTBD14B, NAC1; Nacc1|2010001H03Rik, 4930511N13Rik, Btbd14b, Na, Nac1|nucleus accumbens associated 1, BEN and BTB (POZ) domain containing|nucleus accumbens-associated protein 1|BTB (POZ) domain containing 14B|BTB/POZ domain-containing protein 14B|NAC-1|transcriptional repressor NAC1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NACC1, check out the NACC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NACC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NAC-1 NACC1 Monoclonal Antibody (A08675)
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No publications found for A08675
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-NAC-1 NACC1 Monoclonal Antibody
Question
Would A08675 anti-NAC-1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
You can see on the product datasheet, A08675 anti-NAC-1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-NAC-1 Monoclonal antibody A08675 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The anti-NAC-1 Monoclonal antibody (A08675) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-02
Question
Would anti-NAC-1 Monoclonal antibody A08675 work on primate IHC with leukemic t-cell?
T. Miller
Verified customer
Asked: 2019-08-09
Answer
Our lab technicians have not validated anti-NAC-1 Monoclonal antibody A08675 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NAC-1 Monoclonal antibody A08675 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate leukemic t-cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-NAC-1 Monoclonal antibody A08675. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-25
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-NAC-1 Monoclonal antibody A08675. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-15
Question
Can you help my question with product A08675, anti-NAC-1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08675 anti-NAC-1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-30
Question
We want to test anti-NAC-1 Monoclonal antibody A08675 on human cervix carcinoma for research purposes, then I may be interested in using anti-NAC-1 Monoclonal antibody A08675 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Zhao
Verified customer
Asked: 2016-10-10
Answer
The products we sell, including anti-NAC-1 Monoclonal antibody A08675, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-10-10
