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Product Info Summary
| SKU: | A00372 |
|---|---|
| Size: | 2mL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Myeloperoxidase MPO Antibody
View all Myeloperoxidase/MPO Antibodies
SKU/Catalog Number
A00372
Size
2mL
Form
Lyophilized
Description
Boster Bio Anti-Myeloperoxidase MPO Antibody (Catalog # A00372). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Myeloperoxidase MPO Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00372)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Myeloperoxidase [Human Leukocytes]
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00372 is reactive to MPO in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
Observed Molecular Weight
68 kDa
Calculated molecular weight
45983 MW
Background of Myeloperoxidase/MPO
Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00372 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:200,000
IP: 1:100
WB: 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MPO.
Protein Target Info & Infographic
Gene/Protein Information For MPO (Source: Uniprot.org, NCBI)
Gene Name
MPO
Full Name
Myeloperoxidase
Weight
45983 MW
Superfamily
peroxidase family
Alternative Names
Myeloperoxidase, Myeloperoxidase, MPO MPO myeloperoxidase myeloperoxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MPO, check out the MPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myeloperoxidase MPO Antibody (A00372)
Hello CJ!
A00372 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Myeloperoxidase MPO Antibody
Question
We have tried in the past anti-Myeloperoxidase antibody for WB on liver last year. I am using human, and We intend to use the antibody for IP next. We are interested in examining liver as well as bone marrow cell in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
I looked at the website and datasheets of our anti-Myeloperoxidase antibody and it seems that A00372 has been validated on human in both WB and IP. Thus A00372 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-27
Question
We have observed staining in human plasma. What should we do? Is anti-Myeloperoxidase antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
According to literature plasma does express MPO. According to Uniprot.org, MPO is expressed in bone marrow cell, leukemia, leukocyte, plasma, saliva, liver, among other tissues. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-12-25
Question
Is a blocking peptide available for product anti-Myeloperoxidase antibody (A00372)?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We do provide the blocking peptide for product anti-Myeloperoxidase antibody (A00372). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-01
Question
Would anti-Myeloperoxidase antibody A00372 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
According to the expression profile of leukocyte, MPO is highly expressed in leukocyte. So, it is likely that anti-Myeloperoxidase antibody A00372 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-05-03
Question
My boss were well pleased with the WB result of your anti-Myeloperoxidase antibody. However we have observed positive staining in bone marrow cell lysosome. using this antibody. Is that expected? Could you tell me where is MPO supposed to be expressed?
H. Brown
Verified customer
Asked: 2019-04-02
Answer
From literature, bone marrow cell does express MPO. Generally MPO expresses in lysosome. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-04-02
Question
Will A00372 anti-Myeloperoxidase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
It shows on the product datasheet, A00372 anti-Myeloperoxidase antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-29
Question
We are currently using anti-Myeloperoxidase antibody A00372 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
J. Brown
Verified customer
Asked: 2018-10-01
Answer
The anti-Myeloperoxidase antibody (A00372) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-01
Question
I was wanting to use your anti-Myeloperoxidase antibody for WB for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
As indicated on the product datasheet, A00372 anti-Myeloperoxidase antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-13
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-Myeloperoxidase antibody A00372. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-06
Question
Is there a BSA free version of anti-Myeloperoxidase antibody A00372 available?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Myeloperoxidase antibody A00372 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-23
Question
I see that the anti-Myeloperoxidase antibody A00372 works with WB, what is the protocol used to produce the result images on the product page?
B. Mangal
Verified customer
Asked: 2017-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-06
Question
Can you help my question with product A00372, anti-Myeloperoxidase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00372 anti-Myeloperoxidase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Myeloperoxidase antibody A00372. Let me know if you need anything else.
L. Moore
Verified customer
Asked: 2016-12-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-12-21
Question
you antibody to test anti-Myeloperoxidase antibody A00372 on human leukocyte for research purposes, then I may be interested in using anti-Myeloperoxidase antibody A00372 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Brown
Verified customer
Asked: 2015-11-13
Answer
The products we sell, including anti-Myeloperoxidase antibody A00372, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-11-13
