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Product Info Summary
| SKU: | A00997-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Myelin Protein Zero/MPZ Antibody Picoband®
View all Myelin Protein Zero Antibodies
SKU/Catalog Number
A00997-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myelin Protein Zero/MPZ Antibody Picoband® catalog # A00997-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin Protein Zero/MPZ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00997-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Myelin Protein Zero recombinant protein (Position: I30-R153).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00997-1 is reactive to MPZ in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.555kDa
Background of Myelin Protein Zero
Myelin protein zero (P0, MPZ) is a glycoprotein which in humans is encoded by the MPZ gene. This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in this gene are associated with autosomal dominant form of Charcot-Marie-Tooth disease type 1 (CMT1B) and other polyneuropathies, such as Dejerine-Sottas syndrome (DSS) and congenital hypomyelinating neuropathy (CHN). A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00997-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U-87MG whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Myelin Protein Zero using anti-Myelin Protein Zero antibody (A00997-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myelin Protein Zero antigen affinity purified polyclonal antibody (Catalog # A00997-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myelin Protein Zero at approximately 28KD. The expected band size for Myelin Protein Zero is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For MPZ (Source: Uniprot.org, NCBI)
Gene Name
MPZ
Full Name
Myelin protein P0
Weight
27.555kDa
Superfamily
myelin P0 protein family
Alternative Names
Myelin protein P0; Myelin peripheral protein; MPP; Myelin protein zero; MPZ MPZ CHM, CHN2, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, CMTDID, DSS, HMSNIB, MPP, P0 myelin protein zero myelin protein P0|Charcot-Marie-Tooth neuropathy 1B|myelin peripheral protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MPZ, check out the MPZ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MPZ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myelin Protein Zero/MPZ Antibody Picoband® (A00997-1)
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Customer Q&As
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5 Customer Q&As for Anti-Myelin Protein Zero/MPZ Antibody Picoband®
Question
I see that the anti-Myelin Protein Zero/MPZ antibody A00997-1 works with ELISA, what is the protocol used to produce the result images on the product page?
D. Edwards
Verified customer
Asked: 2020-02-25
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-Myelin Protein Zero/MPZ antibody A00997-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
J. Zhao
Verified customer
Asked: 2019-12-31
Answer
The anti-Myelin Protein Zero/MPZ antibody (A00997-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-31
Question
My question regarding product A00997-1, anti-Myelin Protein Zero/MPZ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00997-1 anti-Myelin Protein Zero/MPZ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-18
Question
I would like to test anti-Myelin Protein Zero/MPZ antibody A00997-1 on mouse skin for research purposes, then I may be interested in using anti-Myelin Protein Zero/MPZ antibody A00997-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The products we sell, including anti-Myelin Protein Zero/MPZ antibody A00997-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-07-03
Question
Is a blocking peptide available for product anti-Myelin Protein Zero/MPZ antibody (A00997-1)?
C. Krishna
Verified customer
Asked: 2013-06-25
Answer
We do provide the blocking peptide for product anti-Myelin Protein Zero/MPZ antibody (A00997-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-06-25
