Click image to see more details
Product Info Summary
| SKU: | M03294 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody
SKU/Catalog Number
M03294
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody catalog # M03294. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03294)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACID-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myelin oligodendrocyte glycoprotein Mediates homophilic cell-cell adhesion (By similarity) . Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03294 is reactive to MOG in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
80 kDa
Calculated molecular weight
58206 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03294 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:100-1:500
Positive Control
WB: mouse brain
Validation Images & Assay Conditions
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Western blot analysis of Myelin oligodendrocyte glycoprotein expression in mouse brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For MOG (Source: Uniprot.org, NCBI)
Gene Name
MOG
Full Name
Myelin-oligodendrocyte glycoprotein
Weight
58206 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Protein disulfide-isomerase A2;5.3.4.1;Pancreas-specific protein disulfide isomerase;PDIp;PDIA2;PDIP; MOG BTN6, BTNL11IG2, NRCLP7, MOG myelin oligodendrocyte glycoprotein myelin-oligodendrocyte glycoprotein|MOG AluA|MOG AluB|MOG Ig-AluB|MOG alpha-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MOG, check out the MOG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MOG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody (M03294)
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Customer Q&As
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15 Customer Q&As for Anti-Myelin oligodendrocyte glycoprotein Monoclonal Antibody
Question
We have been able to see staining in mouse brain. Do you have any suggestions? Is anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Based on literature brain does express MOG. Based on Uniprot.org, MOG is expressed in c1 segment of cervical spinal cord, brain, brain cortex, among other tissues. Regarding which tissues have MOG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7964757, 15489334
Brain cortex, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-13
Question
Will anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 work for IF with brain?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
According to the expression profile of brain, MOG is highly expressed in brain. So, it is likely that anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 will work for IF with brain.
Boster Scientific Support
Answered: 2020-01-13
Question
I was wanting to use your anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody for IF for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
As indicated on the product datasheet, M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-06
Question
Can you help my question with product M03294, anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Is this M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody reactive to the isotypes of MOG?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The immunogen of M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody is A synthesized peptide derived from human Myelin oligodendrocyte glycoprotein Mediates homophilic cell-cell adhesion (By similarity) . Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-17
Question
Would M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
You can see on the product datasheet, M03294 anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-18
Question
Is a blocking peptide available for product anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody (M03294)?
R. Parker
Verified customer
Asked: 2018-11-12
Answer
We do provide the blocking peptide for product anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody (M03294). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-12
Question
I would like to test anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 on mouse brain for research purposes, then I may be interested in using anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Krishna
Verified customer
Asked: 2018-08-27
Answer
The products we sell, including anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-27
Question
I see that the anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-11
Question
We were satisfied with the WB result of your anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody. However we have seen positive staining in brain isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is MOG supposed to be expressed?
D. Yang
Verified customer
Asked: 2017-10-12
Answer
Based on literature, brain does express MOG. Generally MOG expresses in isoform 1: cell membrane. Regarding which tissues have MOG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7964757, 15489334
Brain cortex, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-10-12
Question
We are currently using anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody (M03294) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-02
Question
See below the WB image, lot number and protocol we used for brain using anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-08
Question
We ordered your anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody for WB on brain in the past. I am using human, and We want to use the antibody for IF next. I would like examining brain as well as c1 segment of cervical spinal cord in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
G. Walker
Verified customer
Asked: 2017-04-26
Answer
I looked at the website and datasheets of our anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody and it seems that M03294 has been tested on human in both WB and IF. Thus M03294 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-04-26
Question
Is there a BSA free version of anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 available?
M. Miller
Verified customer
Asked: 2016-05-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myelin oligodendrocyte glycoprotein Monoclonal antibody M03294 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-10
