Anti-Muscleblind Like Protein 1 Monoclonal Antibody

Product Name

Anti-Muscleblind Like Protein 1 Monoclonal Antibody

View all Muscleblind-like 1 Antibodies

SKU/Catalog Number

M02309

Size

100ul

Description

Boster Bio Anti-Muscleblind Like Protein 1 Monoclonal Antibody catalog # M02309. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Muscleblind Like Protein 1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02309)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Immunogen

Full-length recombinant human muscleblind-like 1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02309 is reactive to MBNL1 in Human

Applications

M02309 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41817 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000. ICC/IF or IHC: 1:1,000.

Validation Images & Assay Conditions

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Western blot analysis of whole cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to MBNL1, M02309, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293 cytosol, [3] HEK293 nuclear fraction, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear fraction, [6] HeLa cytosol, and [7] HeLa nuclear fraction. The strong band at the 40kDa corresponds to the MBNL1 protein that is predominantly detected in the nuclear enriched fractions. This antibody binds exclusively to the human protein and does not bind the rodent homologue.

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Immunofluorescent analysis of HeLa cells stained with mouse mAb to MBNL1, M02309, dilution 1:1,000 in green and chicken pAb to HSP60, dilution 1:5,000, in red. The M02309 antibody specifically labels nuclei, while the other antibody reveals protein expressed in mitochondria.

Gene/Protein Information For MBNL1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686P06174; EXP40; EXP42; EXPKIAA0428EXP35; MBNL; muscleblind (Drosophila)-like; muscleblind-like (Drosophila); muscleblind-like protein 1; Triplet-expansion RNA-binding protein MBNL1 EXP, MBNL muscleblind like splicing regulator 1 muscleblind-like protein 1|muscleblind-like|triplet-expansion RNA-binding protein

For more info on MBNL1, check out the MBNL1 Infographic

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In this infographic, you will see the following information for MBNL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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