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Product Info Summary
| SKU: | M02309 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Muscleblind Like Protein 1 Monoclonal Antibody
View all Muscleblind-like 1 Antibodies
SKU/Catalog Number
M02309
Size
100ul
Description
Boster Bio Anti-Muscleblind Like Protein 1 Monoclonal Antibody catalog # M02309. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Muscleblind Like Protein 1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02309)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full-length recombinant human muscleblind-like 1 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02309 is reactive to MBNL1 in Human
Reconstitution
Calculated molecular weight
41817 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02309 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000. ICC/IF or IHC: 1:1,000.
Validation Images & Assay Conditions
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Western blot analysis of whole cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to MBNL1, M02309, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293 cytosol, [3] HEK293 nuclear fraction, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear fraction, [6] HeLa cytosol, and [7] HeLa nuclear fraction. The strong band at the 40kDa corresponds to the MBNL1 protein that is predominantly detected in the nuclear enriched fractions. This antibody binds exclusively to the human protein and does not bind the rodent homologue.
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Immunofluorescent analysis of HeLa cells stained with mouse mAb to MBNL1, M02309, dilution 1:1,000 in green and chicken pAb to HSP60, dilution 1:5,000, in red. The M02309 antibody specifically labels nuclei, while the other antibody reveals protein expressed in mitochondria.
Protein Target Info & Infographic
Gene/Protein Information For MBNL1 (Source: Uniprot.org, NCBI)
Gene Name
MBNL1
Full Name
Muscleblind-like protein 1
Weight
41817 MW
Superfamily
muscleblind family
Alternative Names
Muscleblind-like protein 1;Triplet-expansion RNA-binding protein;MBNL1;EXP, KIAA0428, MBNL; MBNL1 EXP, MBNL muscleblind like splicing regulator 1 muscleblind-like protein 1|muscleblind-like|triplet-expansion RNA-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBNL1, check out the MBNL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBNL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Muscleblind Like Protein 1 Monoclonal Antibody (M02309)
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No publications found for M02309
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Muscleblind Like Protein 1 Monoclonal Antibody
Question
I see that the anti-Muscleblind Like Protein 1 Monoclonal antibody M02309 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-13
Question
Please see the WB image, lot number and protocol we used for testis using anti-Muscleblind Like Protein 1 Monoclonal antibody M02309. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Muscleblind Like Protein 1 Monoclonal antibody M02309. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-28
Question
Would M02309 anti-Muscleblind Like Protein 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
As indicated on the product datasheet, M02309 anti-Muscleblind Like Protein 1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-16
Question
We are currently using anti-Muscleblind Like Protein 1 Monoclonal antibody M02309 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
R. Huang
Verified customer
Asked: 2018-07-20
Answer
The anti-Muscleblind Like Protein 1 Monoclonal antibody (M02309) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-20
