Click image to see more details
Product Info Summary
| SKU: | PA1325-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband®
View all Muscarinic Acetylcholine Receptor M2/CHRM2 Antibodies
SKU/Catalog Number
PA1325-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody catalog # PA1325-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1325-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Muscarinic Acetylcholine Receptor 2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1325-1 is reactive to CHRM2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
51715 MW
Background of Muscarinic Acetylcholine Receptor M2/CHRM2
The muscarinic acetylcholine receptor M2, also known as the cholinergic receptor, muscarinic 2, is a muscarinic acetylcholine receptor that in humans is encoded by the CHRM2 gene. The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1325-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Muscarinic Acetylcholine Receptor 2 antibody, PA1325-1, IHC(P)
IHC(P): Rat Brain Tissue
Click image to see more details
Figure 2. Western blot analysis of Muscarinic Acetylcholine Receptor 2 using anti-Muscarinic Acetylcholine Receptor 2 antibody (PA1325-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Muscarinic Acetylcholine Receptor 2 antigen affinity purified polyclonal antibody (Catalog # PA1325-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Muscarinic Acetylcholine Receptor 2 at approximately 71 kDa. The expected band size for Muscarinic Acetylcholine Receptor 2 is at 52 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CHRM2 (Source: Uniprot.org, NCBI)
Gene Name
CHRM2
Full Name
Muscarinic acetylcholine receptor M2
Weight
51715 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Muscarinic acetylcholine receptor M2;CHRM2; CHRM2 HM2 cholinergic receptor muscarinic 2 muscarinic acetylcholine receptor M2|7TM receptor|acetylcholine receptor, muscarinic 2|muscarinic M2 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRM2, check out the CHRM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband® (PA1325-1)
Hello CJ!
No publications found for PA1325-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody (PA1325-1)?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We do provide the blocking peptide for product anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody (PA1325-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody PA1325-1 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
The anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody (PA1325-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-29
Question
Does anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody PA1325-1 work on zebrafish IHC with brain?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
Our lab technicians have not tested anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody PA1325-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Muscarinic Acetylcholine Receptor 2/CHRM2 antibody PA1325-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-20
