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Product Info Summary
| SKU: | A05052-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Musashi 1/Msi1 Antibody Picoband®
SKU/Catalog Number
A05052-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Musashi 1/Msi1 Antibody Picoband® catalog # A05052-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Musashi 1/Msi1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05052-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Musashi 1/Msi1 recombinant protein (Position: Q33-S347).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05052-2 is reactive to MSI1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39125 MW
Background of Musashi-1
RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05052-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U2OS whole cell, human T-47D whole cell, human COLO-320 whole cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MSI1 using anti-MSI1 antibody (A05052-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human COLO-320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSI1 antigen affinity purified polyclonal antibody (Catalog # A05052-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSI1 at approximately 39KD. The expected band size for MSI1 is at 39KD.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-MSI1 antibody (A05052-2).
Overlay histogram showing A549 cells stained with A05052-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSI1 Antibody (A05052-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MSI1 (Source: Uniprot.org, NCBI)
Gene Name
MSI1
Full Name
RNA-binding protein Musashi homolog 1
Weight
39125 MW
Superfamily
Musashi family
Alternative Names
RNA-binding protein Musashi homolog 1; Musashi-1; MSI1 MSI1 musashi RNA binding protein 1 RNA-binding protein Musashi homolog 1|musashi-1|musashi1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSI1, check out the MSI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Musashi 1/Msi1 Antibody Picoband® (A05052-2)
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