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Product Info Summary
| SKU: | M00612 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat, Cat, Hedgehog |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal Antibody
SKU/Catalog Number
M00612
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal Antibody (Catalog # M00612). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00612)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM297
Isotype
IgG1, kappa
Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M00612 is reactive to MUC5AC in Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat, Cat, Hedgehog
Reconstitution
Reconstitute with distilled water.
Calculated molecular weight
585570 MW
Background of MUC5AC
This monoclonal antibody recognizes the peptide core of gastric mucin M1 (1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00612 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (SPM297).
Protein Target Info & Infographic
Gene/Protein Information For MUC5AC (Source: Uniprot.org, NCBI)
Gene Name
MUC5AC
Full Name
Mucin-5AC
Weight
585570 MW
Alternative Names
Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ; MUC5AC MUC5, TBM, leB, mucin mucin 5AC, oligomeric mucus/gel-forming mucin-5AC|gastric mucin|lewis B blood group |major airway glycoprotein|mucin 5, subtypes A and C, tracheobronchial/gastric|mucin 5AC, oligomeric mucus/gel-forming pseudogene|mucin-5 subtype AC, tracheobronchial|tracheobronchial mucin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MUC5AC, check out the MUC5AC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MUC5AC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal Antibody (M00612)
Hello CJ!
M00612 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Systems pharmacology-based study of Tanreqing injection in airway mucus hypersecretion
Yan Wang,Tian Liu,Jun-fei Wang et al.FSTL1 aggravates OVA-induced allergic airway inflammation by activating NLRP3 inflammasome, 02 April 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-19657/v1]
Species: Human,Mouse
M00612 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:1:250
Wang Q,Wang M,Liu C,Huang L,Gao Y,Yu M,Zhao S,Li X.Ammonia Exposure Induced Cilia Dysfunction of Nasal Mucosa in the Piglets.Biomed Res Int.2020 May 25;2020:1705387.doi:10.1155/2020/1705387.PMID: 32566662;PMCID:PMC7273420.
Species: Pig
M00612 usage in article: APP:IHC, SAMPLE:NASAL MUCOSA, DILUTION:1:100
Wang X,Wang M,Chen S,Wei B,Gao Y,Huang L,Liu C,Huang T,Yu M,Zhao SH,Li X.Ammonia exposure causes lung injuries and disturbs pulmonary circadian clock gene network in a pig study.Ecotoxicol Environ Saf.2020 Dec 1;205:111050.doi:10.1016/j.ecoenv.2020.111050
Species: Pig
M00612 usage in article: APP:IHC, SAMPLE:BRONCHIAL EPITHELIUM, DILUTION:1:100
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal Antibody
Question
Our lab want to know about to test anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 on rat tracheobronchial mucosa for research purposes, then I may be interested in using anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The products we sell, including anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tracheobronchial mucosa using anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-27
Question
I see that the anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-28
Question
We are currently using anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says chicken, human, monkey, mouse, pig, rabbit, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody (M00612) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-13
Question
My boss were satisfied with the WB result of your anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody. However we have observed positive staining in stomach secreted. using this antibody. Is that expected? Could you tell me where is MUC5AC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
According to literature, stomach does express MUC5AC. Generally MUC5AC expresses in secreted. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2019-05-28
Question
I was wanting to use your anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody for IHC for rat tracheobronchial mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat tracheobronchial mucosa identification?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
It shows on the product datasheet, M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody has been tested for IHC on chicken, human, monkey, mouse, pig, rabbit, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tracheobronchial mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-26
Question
Is a blocking peptide available for product anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody (M00612)?
R. Banerjee
Verified customer
Asked: 2019-03-19
Answer
We do provide the blocking peptide for product anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody (M00612). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-19
Question
Would anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 work on dog IHC with stomach?
Verified Customer
Verified customer
Asked: 2018-09-05
Answer
Our lab technicians have not validated anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 on dog. You can run a BLAST between dog and the immunogen sequence of anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog stomach in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-05
Question
Do you have a BSA free version of anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 available?
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-25
Question
Would anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 work for IHC with tracheobronchial mucosa?
M. Jackson
Verified customer
Asked: 2017-02-21
Answer
According to the expression profile of tracheobronchial mucosa, MUC5AC is highly expressed in tracheobronchial mucosa. So, it is likely that anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612 will work for IHC with tracheobronchial mucosa.
Boster Scientific Support
Answered: 2017-02-21
Question
We have observed staining in human colon. What should we do? Is anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody supposed to stain colon positively?
C. Thomas
Verified customer
Asked: 2016-10-17
Answer
From literature colon does express MUC5AC. From Uniprot.org, MUC5AC is expressed in nasal cavity epithelium, trachea, tracheobronchial mucosa, placenta trachea, nasal polyp, colon, stomach, among other tissues. Regarding which tissues have MUC5AC expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Nasal polyp, Pubmed ID: 7513696, 7775418
Placenta, and Trachea, Pubmed ID: 9620876
Stomach, Pubmed ID: 17974005
Trachea, Pubmed ID: 9506983
Tracheobronchial mucosa, Pubmed ID: 2656675
Boster Scientific Support
Answered: 2016-10-17
Question
See below the WB image, lot number and protocol we used for tracheobronchial mucosa using anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody M00612. Please let me know if you require anything else.
N. Johnson
Verified customer
Asked: 2015-11-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-23
Question
Is this M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody reactive to the isotypes of MUC5AC?
M. Zhang
Verified customer
Asked: 2015-05-08
Answer
The immunogen of M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody is M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-05-08
Question
Will M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
W. Dhar
Verified customer
Asked: 2015-03-27
Answer
As indicated on the product datasheet, M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-03-27
Question
Can you help my question with product M00612, anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jha
Verified customer
Asked: 2013-01-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00612 anti-MUC5AC (Mucin 5AC/Gastric Mucin) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-22
