Product Info Summary
SKU: | A02025-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MTHFD1 Antibody Picoband™
SKU/Catalog Number
A02025-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MTHFD1 Antibody Picoband™ catalog # A02025-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MTHFD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02025-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MTHFD1 recombinant protein (Position: K21-Q855).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02025-1 is reactive to MTHFD1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
102 kDa
Calculated molecular weight
37492 MW
Background of MTHFD1
Monofunctional C1-tetrahydrofolate synthase, mitochondrial also known as formyltetrahydrofolate synthetase, is an enzyme that in humans is encoded by the MTHFD1L gene (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like). The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02025-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml/ml, Human
Immunofluorescence, 5 μg/ml/ml, Human
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Human
Direct ELISA, 0.1-0.5 μg/ml/ml, Human
Positive Control
Validation Images & Assay Conditions
![a02025 1 mthfd1 primary antibodies wb testing 1 a02025 1 mthfd1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1 antigen affinity purified polyclonal antibody (Catalog # A02025-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1 at approximately 102 kDa. The expected band size for MTHFD1 is at 102 kDa.
![a02025 1 mthfd1 primary antibodies ihc testing 2 a02025 1 mthfd1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human adenocarcinoma of the lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies ihc testing 3 a02025 1 mthfd1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human chronic tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies ihc testing 4 a02025 1 mthfd1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human diffuse large B cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies ihc testing 5 a02025 1 mthfd1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies ihc testing 6 a02025 1 mthfd1 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies ihc testing 7 a02025 1 mthfd1 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IHC analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a02025 1 mthfd1 primary antibodies if testing 8 a02025 1 mthfd1 primary antibodies if testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-if-testing-8.jpg)
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Figure 8. IF analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a02025 1 mthfd1 primary antibodies fcm testing 10 a02025 1 mthfd1 primary antibodies fcm testing 10](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-fcm-testing-10.jpg)
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Figure 10. Flow Cytometry analysis of JK cells using anti-MTHFD1 antibody (A02025-1).
Overlay histogram showing JK cells stained with A02025-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTHFD1 Antibody (A02025-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a02025 1 mthfd1 primary antibodies if testing 9 a02025 1 mthfd1 primary antibodies if testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a02025-1-mthfd1-primary-antibodies-if-testing-9.jpg)
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Figure 9. IF analysis of MTHFD1 using anti-MTHFD1 antibody (A02025-1).
MTHFD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MTHFD1 Antibody (A02025-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For MTHFD1 (Source: Uniprot.org, NCBI)
Gene Name
MTHFD1
Full Name
C-1-tetrahydrofolate synthase, cytoplasmic
Weight
37492 MW
Alternative Names
C1-THF synthase; EC 6.3.4.3; methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1; MTHFCC-1-tetrahydrofolate synthase, cytoplasmic; MTHFDcytoplasmic C-1-tetrahydrofolate synthase MTHFD1 CIMAH, MTHFC, MTHFD methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 C-1-tetrahydrofolate synthase, cytoplasmic|5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase|C1-THF synthase|cytoplasmic C-1-tetrahydrofolate synthase|epididymis secretory sperm binding protein|methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTHFD1, check out the MTHFD1 Infographic
![MTHFD1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTHFD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MTHFD1 Antibody Picoband™ (A02025-1)
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