Product Info Summary
SKU: | A02292 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MT-ND1 Antibody
SKU/Catalog Number
A02292
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MT-ND1 Antibody (Catalog # A02292). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MT-ND1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02292)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MT-ND1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02292 is reactive to MT-ND1 in Human
Reconstitution
Calculated molecular weight
35661 MW
Background of MT-ND1
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. HAMAP-Rule MF_01350
Anderson S., Nature 290:457-465(1981).
Horai S., Proc. Natl. Acad. Sci. U.S.A. 92:532-536(1995).
Moilanen J.S., Mol. Biol. Evol. 20:2132-2142(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02292 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from A549 cells and COLO cells, using MT-ND1 antibody A02292.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using MT-ND1 antibody A02292.
Protein Target Info & Infographic
Gene/Protein Information For MT-ND1 (Source: Uniprot.org, NCBI)
Gene Name
MT-ND1
Full Name
NADH-ubiquinone oxidoreductase chain 1
Weight
35661 MW
Superfamily
complex I subunit 1 family
Alternative Names
NADH-ubiquinone oxidoreductase chain 1;1.6.5.3;NADH dehydrogenase subunit 1;MT-ND1;MTND1, NADH1, ND1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MT-ND1, check out the MT-ND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MT-ND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MT-ND1 Antibody (A02292)
Hello CJ!
No publications found for A02292
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MT-ND1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MT-ND1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-MT-ND1 Antibody
Question
Does anti-MT-ND1 antibody A02292 work for WB with cerebellum?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
According to the expression profile of cerebellum, MT-ND1 is highly expressed in cerebellum. So, it is likely that anti-MT-ND1 antibody A02292 will work for WB with cerebellum.
Boster Scientific Support
Answered: 2020-02-03
Question
Is this A02292 anti-MT-ND1 antibody reactive to the isotypes of MT-ND1?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The immunogen of A02292 anti-MT-ND1 antibody is Synthesized peptide derived from internal of human MT-ND1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-21
Question
Is a blocking peptide available for product anti-MT-ND1 antibody (A02292)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We do provide the blocking peptide for product anti-MT-ND1 antibody (A02292). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-MT-ND1 antibody A02292 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
The anti-MT-ND1 antibody (A02292) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-09
Question
Will anti-MT-ND1 antibody A02292 work on primate WB with placenta?
Verified Customer
Verified customer
Asked: 2017-05-15
Answer
Our lab technicians have not tested anti-MT-ND1 antibody A02292 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MT-ND1 antibody A02292 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-15
Question
Our lab want to know about to test anti-MT-ND1 antibody A02292 on human cerebellum for research purposes, then I may be interested in using anti-MT-ND1 antibody A02292 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Baker
Verified customer
Asked: 2016-08-16
Answer
The products we sell, including anti-MT-ND1 antibody A02292, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-08-16